Molecular cloning and characterization of prokineticin receptors
| Autor: | Masazumi Kamohara, Kiyoshi Furuichi, Takatoshi Soga, Hideki Hiyama, Takahide Ohishi, Shun Ichiro Matsumoto, Tamaki Oda, Jun Takasaki, Tetsu Saito, Hitoshi Matsushime |
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| Rok vydání: | 2002 |
| Předmět: |
Male
DNA Complementary Receptors Peptide Molecular Sequence Data Biophysics Receptors Cell Surface Molecular cloning Biology Polymerase Chain Reaction Biochemistry Homology (biology) Receptors G-Protein-Coupled Gastrointestinal Hormones Genes Reporter Structural Biology Testis Genetics Humans Amino Acid Sequence RNA Messenger Cloning Molecular Luciferases Receptor G protein-coupled receptor Neuropeptides Brain Prokineticin receptor 2 Prokineticin receptor 1 Prokineticin Molecular biology Cell biology Signal transduction |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 1579:173-179 |
| ISSN: | 0167-4781 |
| Popis: | Recent studies have identified two novel biofunctional proteins, termed prokineticin 1/EG-VEGF and prokineticin 2, which were mammalian homologues of mamba MIT1 and frog Bv8. Prokineticins have been demonstrated to exert their physiological functions through G-protein coupled receptors (GPCRs). In this study, we report the molecular identification of two endogenous prokineticin receptors, designated PK-R1 and PK-R2, through a search of the human genomic DNA database. PK-R1, locating in chromosome 2, and PK-R2, locating in chromosome 20p13, shared 87% homology, which was an extremely high value among known GPCRs. In functional assays, mammalian cells expressing PK-Rs responded to prokineticins in a concentration-dependent manner. Tissue distribution analysis revealed that expression of PK-R1 was observed in the testis, medulla oblongata, skeletal muscle and skin, while that of PK-R2 showed preferential expression in the central nervous system. The tissue distribution of PK-Rs reported in this paper suggests that the prokineticins play multifunctional roles in vivo. |
| Databáze: | OpenAIRE |
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