Urea-stable lactate dehydrogenase as an index of cardiac isoenzymes
| Autor: | Seppo Lindy, Aarne Konttinen |
|---|---|
| Rok vydání: | 1967 |
| Předmět: |
medicine.medical_specialty
Myocardial Infarction Coronary Disease Isozyme chemistry.chemical_compound Internal medicine Lactate dehydrogenase Anemia Pernicious medicine Humans Urea In patient Myocardial infarction pernicious anemia L-Lactate Dehydrogenase business.industry Liver Diseases Myocardium Healthy subjects Acute coronary insufficiency Clinical Enzyme Tests medicine.disease Surgery Isoenzymes Endocrinology Liver chemistry Spectrophotometry Cardiology Cardiology and Cardiovascular Medicine business |
| Zdroj: | The American Journal of Cardiology. 19:563-571 |
| ISSN: | 0002-9149 |
| DOI: | 10.1016/0002-9149(67)90423-7 |
| Popis: | A spectrophotometric test for the determination of cardiac lactic dehydrogenase (LDH) isoenzymes has been presented. This simple test is based on the denaturation of noncardiac isoenzymes by means of urea. The activities of LDH1, heart homogenate-LDH, LDH5 and liver homogenate-LDH were suppressed in 2 M urea to 92, 81, 8 and 10 per cent, respectively. The value of this test as an index of myocardial infarction has been examined. The material consisted of 60 patients with myocardial infarction, 34 suffering from acute coronary insufficiency, 35 with liver diseases, 11 with pernicious anemia, and 43 healthy subjects. Serum LDH activity was suppressed in urea solution to 74 ± 5 per cent in the control group, to 91±7 per cent in patients with myocardial infarction and to 40 ± 33 per cent in patients with liver diseases, being in the latter much less (11 and 14 per cent) in the cases with the greatest total activities. The differences between these three groups are highly significant. The urea-stable LDH percentage, which depends on the subunit composition of serum isoenzymes, refers to the organ damaged, while serum total LDH reflects the extent of the injury. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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