l ‐Methionine activates Nrf2‐ARE pathway to induce endogenous antioxidant activity for depressing ROS‐derived oxidative stress in growing rats
| Autor: | Hui Li, Liang Cai, Mingcai Liang, Qiong Wu, Zhengxuan Wang, Hongjuan He, Lin Yang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Male
NF-E2-Related Factor 2 030309 nutrition & dietetics Glutathione reductase Antioxidants 03 medical and health sciences chemistry.chemical_compound Methionine 0404 agricultural biotechnology Animals Rats Wistar chemistry.chemical_classification Glutathione Peroxidase 0303 health sciences Kelch-Like ECH-Associated Protein 1 Nutrition and Dietetics biology Glutathione peroxidase 04 agricultural and veterinary sciences Glutathione 040401 food science Glutathione synthase Rats Oxidative Stress Liver chemistry Biochemistry Methionine Adenosyltransferase biology.protein Methionine sulfoxide reductase Reactive Oxygen Species Agronomy and Crop Science Food Science Biotechnology MSRA |
| Zdroj: | Journal of the Science of Food and Agriculture. 99:4849-4862 |
| ISSN: | 1097-0010 0022-5142 |
| DOI: | 10.1002/jsfa.9757 |
| Popis: | Background Methionine is an essential sulfur-containing amino acid. To elucidate the influence of l-methionine on activation of the nuclear factor erythroid 2-related factor 2-antioxidant responsive element (Nrf2-ARE) antioxidant pathway to stimulate the endogenous antioxidant activity for depressing reactive oxygen species (ROS)-derived oxidative stress, male Wistar rats were orally administered l-methionine daily for 14 days. Results With the intake of l-methionine, Nrf2 was activated by l-methionine through depressing Keap1 and Cul3, resulting in upregulation of ARE-driven antioxidant expression (glutamate cysteine ligase catalytic subunit, glutamate cysteine ligase modulatory subunit, glutathione synthase (GS), catalase (CAT), superoxide dismutase (SOD), heme oxygenase 1, NAD(P)H:quinone oxidoreductase 1, glutathione reductase (GR), glutathione S-transferase (GST), glutathione peroxidase (GPx)) with increasing l-methionine availability. Upon activation of Nrf2, glutathione synthesis was increased through upregulated expression of methionine adenosyltransferase, S-adenosylhomocysteine hydrolase, cystathionine β-synthase, cystathionine γ-lyse, glutamate cysteine ligase (GCL) and GS, while hepatic expressions of methionine sulfoxide reductases (MsrA, MsrB2, MsrB3) and hepatic enzyme activities (CAT, SOD, GCL, GR, GST, GPx) were uniformly stimulated with increasing consumption of l-methionine. As a result, hepatic content of ROS and MDA were effectively reduced by l-methionine intake. Conclusion The present study demonstrates that methionine availability plays a critical role in activation of the Nrf2-ARE pathway to induce an endogenous antioxidant response for depressing ROS-derived oxidative stress, which is primarily attributed to the stimulation of methionine sulfoxide reductase expression and glutathione synthesis. © 2019 Society of Chemical Industry. |
| Databáze: | OpenAIRE |
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