Study of flow rate in pressurized gradient capillary electrochromatography using splitter and separation of peptides using an Amide stationary phase
| Autor: | Tatsunari Yoshida, Rie Nakashima, Takao Tsuda, Shinya Kitagawa |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Capillary electrochromatography
Chromatography biology Capillary action Chemistry Hydrophilic interaction chromatography Organic Chemistry Analytical chemistry General Medicine Amides Biochemistry Analytical Chemistry Volumetric flow rate Electrophoresis Electrochromatography Phase (matter) Pressure biology.protein Bovine serum albumin Peptides Chromatography Micellar Electrokinetic Capillary |
| Zdroj: | Journal of Chromatography A. 1044:305-309 |
| ISSN: | 0021-9673 |
| Popis: | A pressurized gradient capillary electrochromatograph using a splitter was constructed. The variation in flow rate during gradient elution was investigated and separations of peptides using an Amide stationary phase were demonstrated. The flow rate, which is one of the important factors to control chromatographic behavior, was increased during the gradient elution, and the mismatching of mobile phase between the column and the resistance tubing derived three variation patterns in the flow rate. The electrophoretic migration in electrochromatography could enhance in separation of peptides. The separated peak number of tryptic digest of bovine serum albumin was increased from 30 to 40 by the application of +5 kV. |
| Databáze: | OpenAIRE |
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