Modification of Fully Automated Total Iron-binding Capacity (TIBC) Assay in Serum and Comparison with Dimension TIBC Method
| Autor: | Yoshinori Iwatani, Hachiro Yamanishi, Yoshihisa Yamaguchi, Yuzuru Kanakura, Shigeru Iyama |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
chemistry.chemical_classification
Autoanalysis Chromatography medicine.diagnostic_test Transferrin saturation Iron Biochemistry (medical) Clinical Biochemistry Analytical chemistry Blood Proteins Standard solution chemistry Fully automated Transferrin Total iron-binding capacity Serum iron medicine Automated analyzer Humans Quantitative analysis (chemistry) Protein Binding |
| Zdroj: | Clinical Chemistry. 48:1565-1570 |
| ISSN: | 1530-8561 0009-9147 |
| DOI: | 10.1093/clinchem/48.9.1565 |
| Popis: | Background: We previously reported the development of a fully automated assay for total iron-binding capacity (TIBC) in serum, using a multipurpose automated analyzer. However, this method requires four different reagents and is thus useful only with a limited number of available analyzers. We simplified our original assay and compared the analytical performance of the modified method with that of a commercial, fully automated TIBC assay (Dimension® TIBC assay).Methods: We simplified our original method to require only three reagents. Calibration was also altered and was performed with human transferrin standard solutions. An advantage of this method is that it does not require separation of excess unbound iron after the first step of transferrin saturation. Unbound iron is eliminated by formation of a complex with the chromogenic reagent ferrozine in the second step. Iron dissociated from transferrin by acidic pH reacts with ferrozine to form a colored complex in the final step, and the increase in absorbance at 570/660 nm is directly proportional to the TIBC measured. TIBC values were determined for 49 healthy individuals and 148 patients with this modified TIBC assay and with a commercial, fully automated TIBC method (Dimension clinical chemistry system), and calculation of TIBC based on the sum of the serum iron and unsaturated iron-binding capacity was performed for 97 patients.Results: The within-run CVs for the modified TIBC assay and the Dimension TIBC assay were Conclusions: Our modified, fully automated TIBC assay performed similarly to the Dimension TIBC assay and is adaptable for use with many multipurpose automated analyzers. |
| Databáze: | OpenAIRE |
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