Solution structure of a DNA double helix with consecutive metal-mediated base pairs
| Autor: | Dominik Böhme, Nicole Megger, Jens Müller, Silke Johannsen, Roland K. O. Sigel |
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| Přispěvatelé: | University of Zurich, Johannsen, S |
| Rok vydání: | 2009 |
| Předmět: |
10120 Department of Chemistry
Models Molecular Magnetic Resonance Spectroscopy Silver Base pair Stereochemistry General Chemical Engineering 1600 General Chemistry chemistry.chemical_compound 540 Chemistry Nucleotide A-DNA 1500 General Chemical Engineering Base Pairing chemistry.chemical_classification Base Sequence Oligonucleotide Imidazoles General Chemistry Nuclear magnetic resonance spectroscopy DNA Solutions Crystallography chemistry Oligodeoxyribonucleotides Helix Nucleic acid |
| Zdroj: | Nature chemistry. 2(3) |
| ISSN: | 1755-4349 |
| Popis: | Metal-mediated base pairs represent a powerful tool for the site-specific functionalization of nucleic acids with metal ions. The development of applications of the metal-modified nucleic acids will depend on the availability of structural information on these double helices. We present here the NMR solution structure of a self-complementary DNA oligonucleotide with three consecutive imidazole nucleotides in its centre. In the absence of transition-metal ions, a hairpin structure is adopted with the artificial nucleotides forming the loop. In the presence of Ag(i) ions, a duplex comprising three imidazole-Ag(+)-imidazole base pairs is formed. Direct proof for the formation of metal-mediated base pairs was obtained from ¹J(¹⁵N,¹⁰⁷/¹⁰⁹Ag) couplings upon incorporation of ¹⁵N-labelled imidazole. The duplex adopts a B-type conformation with only minor deviations in the region of the artificial bases. This work represents the first structural characterization of a metal-modified nucleic acid with a continuous stretch of metal-mediated base pairs. |
| Databáze: | OpenAIRE |
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