Effects of MDMA (ecstasy) on apoptosis and heat shock protein (HSP70) expression in adult rat testis
| Autor: | Fahimeh Mobaraki, Masoumeh Seghatoleslam, Alireza Fazel, Alireza Ebrahimzadeh-Bideskan |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Substance-Related Disorders Health Toxicology and Mutagenesis N-Methyl-3 4-methylenedioxyamphetamine Ecstasy Apoptosis Toxicology 03 medical and health sciences Random Allocation 0302 clinical medicine Internal medicine Heat shock protein Testis Weight Loss medicine In Situ Nick-End Labeling Animals HSP70 Heat-Shock Proteins Rats Wistar Hsp70 expression Dose-Response Relationship Drug Chemistry Gene Expression Regulation Developmental Leydig Cells MDMA Organ Size Immunohistochemistry Hsp70 030104 developmental biology Endocrinology Seminiferous Epithelium Hallucinogens 030217 neurology & neurosurgery Injections Intraperitoneal medicine.drug Body Temperature Regulation |
| Zdroj: | Toxicology mechanisms and methods. 28(3) |
| ISSN: | 1537-6524 |
| Popis: | This study was conducted to investigate the effects of MDMA (3,4-methylenedioxymethamphetamine, ecstasy) on apoptosis and heat shock protein expression in adult rat testis.Twenty male rats were divided into four groups, two experimental groups (1 and 2), sham control and control. For 16 consecutive days, the experimental groups 1 and 2 were received 5 and 10 mg/kg intraperitoneal (ip) injection of ecstasy, respectively, and in the sham control group, the only saline was injected. In the control group there was no intervention. Finally, the rat's testes were removed and processed for terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) and immunohistochemical techniques.Both doses of MDMA in experimental groups 1 and 2 significantly increased the mean number of TUNEL-positive cells in the germinal epithelium and Leydig cells (p 0.05). Also in the experimental groups, the immunoreactivity of heat shock protein 70 (HSP70) significantly increased in the testis (p 0.05).The findings revealed that MDMA administration increases the level of immunoreactivity of HSP70 and TUNEL-positive cells in the testicular tissue. |
| Databáze: | OpenAIRE |
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