Microfluidic Cell Culturing Platform Combining Long-term, High-resolution Imaging with Impedance Spectroscopy
| Autor: | Sebastian C. Bürgel, Olivier Frey, Fabian Rudolf, Gregor W. Schmidt, Andreas Hierlemann, Ketki Chawla |
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| Přispěvatelé: | Urban, Gerald, Wöllenstein, Jürgen, Kieninger, Jochen |
| Rok vydání: | 2015 |
| Předmět: |
0303 health sciences
Materials science Microfluidics 010401 analytical chemistry Impedance spectroscopy Nanotechnology General Medicine 01 natural sciences Yeast Clamping 0104 chemical sciences Dielectric spectroscopy 03 medical and health sciences Cell culture Microscopy Electrical impedance spectroscopy High resolution imaging Layer (electronics) Engineering(all) 030304 developmental biology Biomedical engineering |
| Zdroj: | Procedia Engineering, 120 Eurosensors 2015 |
| ISSN: | 1877-7058 |
| Popis: | We present an integrated microfluidic system, which combines long-term cell culturing with electrical impedance spectroscopy (EIS) on a single chip. Non-adherent cells, such as yeast (S. cerevisiae), can be grown under constant media perfusion in a 2D layer under clamping pads that enable long-term time-lapse microscopy within the same focal plane. The addition of EIS provides a complementary method for continuous in-situ analysis of single cells and has been used here to monitor the growth rate of cell colonies in real time. Procedia Engineering, 120 Eurosensors 2015 |
| Databáze: | OpenAIRE |
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