Magnetic Resonance Imaging of Experimental Inflammatory Bowel Disease
| Autor: | Anja A. Kühl, Christoph Loddenkemper, Birgit Hotz, Bernd Misselwitz, Steffi Valdeig, Bernd Frericks, Karl-Jürgen Wolf, Frank Wacker, Jörg C. Hoffmann |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Pathology medicine.medical_specialty Dinitrobenzene Iron Rat model Iron oxide Contrast Media Ferric Compounds Sensitivity and Specificity Inflammatory bowel disease chemistry.chemical_compound Image Interpretation Computer-Assisted medicine Animals Humans Radiology Nuclear Medicine and imaging Colitis Magnetite Nanoparticles medicine.diagnostic_test business.industry Reproducibility of Results Dextrans Oxides Histology Magnetic resonance imaging General Medicine Mononuclear phagocyte system Image Enhancement medicine.disease Magnetic Resonance Imaging Ferrosoferric Oxide Rats Disease Models Animal chemistry Rats Inbred Lew Injections Intravenous Colitis Ulcerative business |
| Zdroj: | Investigative Radiology. 44:23-30 |
| ISSN: | 0020-9996 |
| DOI: | 10.1097/rli.0b013e3181899025 |
| Popis: | OBJECTIVES To quantitatively and qualitatively characterize the MR findings of inflammatory bowel disease in a rat model after i.v. injection of the reticuloendothelial system cell specific ultrasmall iron oxide SHU 555 C. MATERIALS AND METHODS Colitis was induced in 15 rats using dinitrobenzene sulfonic acid instillation. Five rats served as controls. T1- and T2-weighted spin-echo- and T2*-weighted gradient-echo-sequences were acquired at 2.4 Tesla before and immediately, 15, 45, 60, and 90 minutes, and 24 hours after i.v.-injection of SHU 555 C (0.1 mmol Fe/kg). MR images were evaluated quantitatively regarding thickness and signal-to-noise ratio (SNR) of the bowel wall and qualitatively regarding overall bowel wall signal intensity and the occurrence of bowel wall ulcerations. MR findings were correlated to histology. RESULTS The inflamed bowel wall was significantly thicker than the noninflamed bowel wall and 90 minutes after contrast injection it showed a significant reduction of SNR in T1- (94 +/- 27 vs. 61 +/- 29; P < 0.01), T2- (67 +/- 26 vs. 28 +/- 17; P < 0.05), and T2*- (92 +/- 57 vs. 10 +/- 7; P < 0.05) weighted images as compared with unenhanced images. At 24 hours, the respective SNR values remained significantly reduced. The signal loss was homogeneous in 12 and focal in 3 of the 15 rats with colitis. Nine rats showed colonic wall ulcerations. In all but one animal (missed focal ulceration) MR findings correlated to the histologic findings. CONCLUSIONS SHU 555 C leads to a significant signal intensity loss of the inflamed bowel wall in T1-, T2- and T2*-weighted images. SHU 555 C enhanced MRI findings correlate well with histologic findings. |
| Databáze: | OpenAIRE |
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