Detection of autoimmune antibodies in localized scleroderma by synthetic oligonucleotide antigens

Autor: Kathryn S. Torok, Christian Damsgaard Jørgensen, Simone V Samuelsen, Kira Astakhova, Elizabeth D. Mellins
Rok vydání: 2018
Předmět:
0301 basic medicine
Physiology
Oligonucleotides
lcsh:Medicine
Antigen Processing and Recognition
Biochemistry
Scleroderma
Scleroderma
Localized
030207 dermatology & venereal diseases
chemistry.chemical_compound
0302 clinical medicine
Immune Physiology
Medicine and Health Sciences
Enzyme-Linked Immunoassays
lcsh:Science
Oligonucleotides/chemistry
Connective Tissue Diseases
Localized Scleroderma
Immune System Proteins
Multidisciplinary
integumentary system
biology
Chemistry
Antibody
Research Article
Immunology
Dermatology
Research and Analysis Methods
Skin Diseases
Autoimmune Diseases
03 medical and health sciences
Rheumatology
Antigen Isotypes
Antigen
Diagnostic Medicine
medicine
Humans
Antigens/chemistry
Antigens
Immunoassays
Autoantibodies/analysis
Autoantibodies
Oligonucleotide
lcsh:R
Biology and Life Sciences
Proteins
RNA
Scleroderma
Localized/immunology
medicine.disease
030104 developmental biology
Immunologic Techniques
Nucleic acid
biology.protein
lcsh:Q
Clinical Immunology
Clinical Medicine
Biomarkers
DNA
Zdroj: PLoS ONE
Samuelsen, S, Jørgensen, C D, Mellins, E D, Torok, K S & Astakhova, K 2018, ' Detection of autoimmune antibodies in localized scleroderma by synthetic oligonucleotide antigens ', PLOS ONE, vol. 13, no. 4, e0195381 . https://doi.org/10.1371/journal.pone.0195381
PLoS ONE, Vol 13, Iss 4, p e0195381 (2018)
Samuelsen, S, Jørgensen, C D, Mellins, E D, Torok, K S & Astakhova, K 2018, ' Detection of autoimmune antibodies in localized scleroderma by synthetic oligonucleotide antigens ', P L o S One, vol. 13, no. 4, e0195381 . https://doi.org/10.1371/journal.pone.0195381
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0195381
Popis: In this study, we developed a series of synthetic oligonucleotides that allowed us to investigate the details on the antigen recognition by autoimmune antibodies in localized scleroderma subjects. Besides dramatically improved analytical specificity of the assay, our data suggests a potential linking for antibodies to DNA to the biological status of disease state in localized scleroderma. Moreover, introducing chemical modifications into short synthetic deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) molecules completely changed the binding titers of corresponding antibodies and their clinical relevance. The strongest observed effect was registered for the localized scleroderma skin damage index (LoSDI) on the IgG antibodies to TC dinucleotide-rich double-stranded antigen (p < 0.001). In addition to providing valuable tools for diagnosis of clinically relevant biomarkers, we believe that this work opens up new opportunities for research on antibodies to nucleic acids in localized scleroderma and other autoimmune diseases.
Databáze: OpenAIRE
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