HELQ reverses the malignant phenotype of osteosarcoma cells via CHK1-RAD51 signaling pathway
| Autor: | Ai Fen Peng, Xin Hua Long, Zhi Li Liu, Dong Ning Liu, Xuan Yin Chen, Yun Fei Zhou, Hong Xia |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cancer Research DNA Repair DNA repair Cell RAD51 Bone Neoplasms Biology 03 medical and health sciences Cell Movement Cell Line Tumor medicine Humans Cell Proliferation Osteosarcoma Osteoblasts Oncogene DNA Helicases General Medicine Transfection Cell cycle Molecular biology Gene Expression Regulation Neoplastic Comet assay 030104 developmental biology medicine.anatomical_structure Real-time polymerase chain reaction Oncology Checkpoint Kinase 1 Cancer research Rad51 Recombinase Signal Transduction |
| Zdroj: | Oncology Reports. 37:1107-1113 |
| ISSN: | 1791-2431 1021-335X |
| DOI: | 10.3892/or.2016.5329 |
| Popis: | HELQ is a DNA helicase important for repair of DNA lesions and has been linked to several types of cancer. However, little is known about its relationship with osteosarcoma (OS) and its mechanism. In the present study, the expression of HELQ and its downstream mediators in OS cells was assayed by quantitative PCR and western blot analysis. The function of HELQ in OS cells was investigated by Transwell invasion, wound healing, CCK8 assays and Comet assay. The results demonstrated that HELQ gene and protein were expressed in OS cells. OS cell invasion, migration, proliferation and DNA damage repair were enhanced by HELQ knock-down with shRNA-lentivirus and inhibited by HELQ overexpression with lentivirus transfection. Furthermore, the antitumor activities of HELQ may be associated with upregulated expression of the DNA damage-related proteins CHK1 and RAD51. Our findings indicated that HELQ confers an anti-invasive phenotype on OS cells by activating the CHK1-RAD51 signaling pathway and suggested that HELQ could be recognized as a promising therapeutic target for OS and other types of malignant tumors. |
| Databáze: | OpenAIRE |
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