Identification of Potential Bacteria on Several Lakes in East Java, Indonesia Based on 16S rRNA Sequence Analysis
| Autor: | Mastika Marisahani Ulfah, Uun Rohmawati, Sitoresmi Prabaningtyas, Dwi Listyorini, Eko Agus Suyono, Achmad Rodiansyah, Ainul Fitria Mahmudah |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Bacillus (shape)
Genetics biology Bacillus amyloliquefaciens QH301-705.5 Bacillus cereus Bacillus subtilis biology.organism_classification 16S ribosomal RNA General Biochemistry Genetics and Molecular Biology Genetic distance Biology (General) General Agricultural and Biological Sciences Enterobacter cloacae Bacteria |
| Zdroj: | Hayati Journal of Biosciences, Vol 28, Iss 2 (2021) |
| ISSN: | 2086-4094 1978-3019 |
| Popis: | Four bacterial isolates from Ranu Pani and Ranu Grati in east java had been revealed to be potentials to produce IAA (PIS isolate), phosphate solubilizer (GPS isolate), cellulose hydrolysis (PSS isolate) and, amylum hydrolysis (PAS), two dominant bacterial isolates from Rani Pani (PØD isolate) and Ranu Grati (GØD isolate) which were co-cultured with microalgae promoted microalgae growth, yet its taxonomical position has not been clearly known. The aim of this study was to identify those bacterial isolates using 16S rRNA barcode. This research conducted by gDNA isolation, the 16S rRNA sequence was amplified using 27F and 1492R primers. Reconstructed phylogenetic trees and genetic distance analysis showed that the isolate PIS and PSS identified as Bacillus cereus Group closely related to Bacillus paramycoides. PAS isolate identified as Bacillus subtilis Group closely related to Bacillus amyloliquefaciens, GPS isolate identified as novel species in genus Enterobacter, and two dominant isolates (PØD and GØD) identified as Enterobacter cloacae complex closely related to Enterobacter cloacae. The genomic approach and additional phenotypes-examination are required to clarify its taxonomical position. |
| Databáze: | OpenAIRE |
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