Genistein Alleviates Oxidative Stress and Inflammation in the Hypothalamic Paraventricular Nucleus by Activating the Sirt1/Nrf2 Pathway in High Salt-Induced Hypertension
| Autor: | Li-Gang Niu, Na Sun, Kai-Li Liu, Qing Su, Jie Qi, Li-Yan Fu, Guo-Rui Xin, Yu-Ming Kang |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
Male
Inflammation Niacinamide Glutathione Disulfide NF-E2-Related Factor 2 Superoxide Dismutase Cardiomegaly Toxicology Genistein Antioxidants Rats Oxidative Stress Sirtuin 1 Hypertension Animals Cytokines Rats Wistar Sodium Chloride Dietary Cardiology and Cardiovascular Medicine Reactive Oxygen Species Molecular Biology Paraventricular Hypothalamic Nucleus |
| Zdroj: | Cardiovascular toxicology. 22(10-11) |
| ISSN: | 1559-0259 |
| Popis: | Hypertension caused by a high-salt (HS) diet is one of the major causes of cardiovascular diseases. Underlining pathology includes oxidative stress and inflammation in the hypothalamic paraventricular nucleus (PVN). This study investigates genistein's (Gen) role in HS-induced hypertension and the underlying molecular mechanism. We placed male Wistar rats on HS (8% NaCl) or normal salt diet (0.3% NaCl). Then, we injected bilateral PVN in rats with Gen, vehicle, or nicotinamide (NAM) for 4 weeks. Tail cuff was used weekly to assess the systolic pressure, diastolic pressure, and mean arterial pressure (MAP). Cardiac hypertrophy was analyzed by heart weight/body weight ratio and wheat germ agglutinin staining. ELISA kits, Western blot, or dihydroethidium staining determined the levels of inflammatory cytokines and oxidative stress markers. Western blot measured protein levels of Sirt1, Ac-FOXO1, Nrf2, NQO-1, HO-1, and gp91 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink