Protein structure preservation by MWCNTs/RTIL nano-composite
| Autor: | Hedayatollah Ghourchian, Aghdas Banaei, Somayeh Karimi |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Circular dichroism
Supramolecular chemistry Ionic Liquids Conjugated system Electrochemistry Biochemistry Protein Structure Secondary Nanocomposites Glucose Oxidase Hemoglobins chemistry.chemical_compound Protein structure Structural Biology Humans Organic chemistry Glucose oxidase Electrodes Molecular Biology biology Nanotubes Carbon Chemistry Circular Dichroism Imidazoles Temperature General Medicine Fluorescence Carbon Spectrometry Fluorescence Chemical engineering Ionic liquid biology.protein Spectrophotometry Ultraviolet Aspergillus niger Glass |
| Zdroj: | International Journal of Biological Macromolecules. 56:169-174 |
| ISSN: | 0141-8130 |
| Popis: | The structure changes of hemoglobin (Hb) and glucose oxidase (GOx), as two typical proteins, upon interaction with MWCNTs and MWCNTs-[BMIM]BF₄(-) was studied. The ability of MWCNTs and MWCNTs-([BMIM]BF₄(-)) for the achievement of direct electrochemistry of GOx and Hb and enhancing the electron transfer rate between proteins and electrode surface was compared. Also the data obtained by various techniques including UV-vis, fluorescence and circular dichroism spectroscopy revealed that the structure of Hb or GOx conjugated with supramolecular network of the nano-composite could be preserved much better than those absorbed on MWCNTs. It seems that ionic liquid provides a favorable microenvironment to maintain the protein native structures. |
| Databáze: | OpenAIRE |
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