Hemorrhage Attenuates Neutrophil Recruitment in Response to Secondary Respiratory Infection by Pseudomonas Aeruginosa
| Autor: | Yaping Chen, Joanne Lomas-Neira, Amanda M. Jamieson, Alfred Ayala, Kayla Lee, Runping Zhao, Craig T. Lefort, Zachary S. Wilson, Chun-Shiang Chung, Joshua T. Cohen |
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| Rok vydání: | 2018 |
| Předmět: |
Resuscitation
Neutrophils Secondary infection Chemokine CXCL1 Hemorrhage 030204 cardiovascular system & hematology Critical Care and Intensive Care Medicine medicine.disease_cause Article 03 medical and health sciences Mice 0302 clinical medicine medicine Animals Pseudomonas Infections Interleukin 6 Lung Respiratory Tract Infections Inhalation medicine.diagnostic_test biology Pseudomonas aeruginosa business.industry Interleukin-6 Respiratory infection 030208 emergency & critical care medicine medicine.anatomical_structure Bronchoalveolar lavage Immunology Emergency Medicine biology.protein business |
| Zdroj: | Shock |
| ISSN: | 1540-0514 |
| Popis: | Neutrophil recruitment into the lung airspaces plays an important role in the containment and clearance of bacteria. Hemorrhagic shock, a complication of traumatic injury, induces immune dysfunction that compromises host defense and frequently leads to secondary infection. The objective of the current study was to determine whether prior hemorrhage impacts neutrophil recruitment in response to secondary Pseudomonas aeruginosa. Experiments were performed using a mouse model (C57BL/6) of respiratory infection by P. aeruginosa (strain PA103, 3 × 10(5) colony-forming units [CFUs]) that is delivered by intratracheal inhalation 24 hours after hypovolemic hemorrhagic shock (fixed mean arterial blood pressure at 35 mmHg for 90 minutes, Ringer’s lactate infused as fluid resuscitation). By post-mortem flow cytometry analyses of bronchoalveolar lavage fluid, we observe that prior hemorrhage attenuates the entry of neutrophils into the lung airspaces in response to P. aeruginosa. The reduction in neutrophil recruitment occurs in an amplified inflammatory environment, with elevated lung tissue levels of interleukin 6 and C-X-C motif ligand 1 (CXCL1) in mice receiving hemorrhage prior to infection. As compared to either insult alone, outcome to sequential hemorrhage and respiratory infection includes enhanced mortality compared to either insult alone. The effect of prior hemorrhage on clearance of P. aeruginosa, as determined by quantifying bacterial CFUs in lung tissue, was not statistically significant at 24 hours post-infection, but our data suggest that further inquiry may be needed to fully understand the potential impact of hemorrhagic shock on this process. These results suggest that changes in neutrophil recruitment may contribute to the immune dysfunction following hemorrhagic shock that renders the host susceptible to severe respiratory infection. |
| Databáze: | OpenAIRE |
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