Molecular Characterization of the Host-Adapted Pathogen Verticillium longisporum on the Basis of a Group-I Intron Found in the Nuclear SSU-rRNA Gene
| Autor: | Vassiliki K. Karapapa, Milton A. Typas |
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| Rok vydání: | 2001 |
| Předmět: |
Sequence analysis
Genes Fungal Molecular Sequence Data Brassica Verticillium Polymerase Chain Reaction Applied Microbiology and Biotechnology Microbiology Conserved sequence Verticillium longisporum DNA Ribosomal Spacer Cloning Molecular DNA Fungal Gene Phylogeny Genetics Base Sequence Phylogenetic tree biology fungi Intron Genetic Variation food and beverages Genes rRNA Sequence Analysis DNA General Medicine biology.organism_classification Introns Nucleic Acid Conformation DNA Intergenic Verticillium wilt |
| Zdroj: | Current Microbiology. 42:217-224 |
| ISSN: | 1432-0991 0343-8651 |
| DOI: | 10.1007/s0028403380 |
| Popis: | Verticillium wilt of oilseed rape is caused by the host-adapted pathogen Verticillium longisporum comb. nov. With one set of nuclear SSU-rRNA gene primers, a PCR amplification product of ca. 2.5 kb was generated from all isolates of V. longisporum tested (36 from Europe, Japan, and USA), with the exception of two recombinant isolates. On the contrary, all the other phytopathogenic and non-phytopathogenic species of Verticillium tested (18 species, 46 isolates), with the exception of one isolate of V. lecanii and two of Cordyceps sp., generated a product of ca. 1.65 kb. Sequence analysis of the SSU-rRNA gene of two typical isolates of V. longisporum (wild radish, Japan, and oilseed rape, Germany) revealed that this dimorphism was due to the presence of an identical 839-bp intron located in a highly conserved insertion position (nt 1165 of Saccharomyces cerevisiae). The intron sequence was classified as group-I intron on the basis of conserved sequence and secondary structural elements. Primers designed from the 839-bp intron sequence amplified only the V. longisporum. Phylogenetic analysis based on SSU-rDNA sequences showed that V. longisporum was closely related to the genera of other filamentous Ascomycetes with fruiting bodies. |
| Databáze: | OpenAIRE |
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