Exercise reduces hyperlipidemia‑induced kidney damage in apolipoprotein E‑deficient mice
| Autor: | Hongyang Liu, Xi You, Chengsi Qian, Hupei Zhu, Lipeng Guo, Yan Sun, Qin Yang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Apolipoprotein E Cancer Research medicine.medical_specialty Apolipoprotein B Normal diet medicine.disease_cause 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Immunology and Microbiology (miscellaneous) Internal medicine Hyperlipidemia hyperlipidemia Medicine ApoE-/- mice chemistry.chemical_classification Creatinine exercise biology business.industry Glutathione peroxidase Articles General Medicine medicine.disease kidney damage 030104 developmental biology Endocrinology chemistry 030220 oncology & carcinogenesis biology.protein business TC Oxidative stress Kidney disease |
| Zdroj: | Experimental and Therapeutic Medicine |
| ISSN: | 1792-1015 1792-0981 |
| Popis: | Hyperlipidemia is a risk factor of kidney damage that can lead to chronic kidney disease. Studies have shown that exercise reduces kidney damage; however, the specific mechanisms underlying the protective effects of exercise remain unclear. For 12 weeks, 8-week-old male apolipoprotein E-deficient (ApoE-/-) mice were randomly divided into four treatment groups (n=7/group) as follows: Mice fed a normal diet (ND group); mice fed a ND and exercised (ND + E group); mice fed a high-fat diet (HD group); and mice fed a HD and exercised (HD + E group). Exercise training consisted of swimming for 40 min, 5 days/week. Metabolic parameters, such as low-density lipoprotein-cholesterol, total cholesterol and creatinine levels were higher in the ApoE-/- HD mice compared with those in the ApoE-/- HD + E mice. Serum levels of glutathione peroxidase and superoxide dismutase were significantly decreased in the HD group compared with those in the HD + E group. Significant pathological changes were observed in the HD + E group compared with in the HD group. Immunohistochemistry and immunoblotting revealed increased levels of oxidative stress (nuclear factor erythroid-2-related factor 2) and fibrosis (Smad3 and TGF-β) markers in the ApoE-/- HD group; however, the expression levels of these markers were significantly decreased in the ApoE-/- HD + E group. Furthermore, NF-κB expression in the HD + E group was significantly lower compared with that in the HD group. These results suggested that exercise may exert protective effects against kidney damage caused by hyperlipidemia. |
| Databáze: | OpenAIRE |
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