In vivo and in vitro Effects of Substance P on the Release of β-Endorphin-Like Immunoreactivity
| Autor: | Shiro Saito, Akira Yamanoi, Shigeru Yamamoto, Mitsuhiro Matsumura |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Male
medicine.medical_specialty Endocrinology Diabetes and Metabolism chemistry.chemical_element Substance P Stimulation Calcium Ouabain Cellular and Molecular Neuroscience chemistry.chemical_compound Endocrinology Anterior pituitary Pituitary Gland Anterior In vivo Culture Techniques Internal medicine medicine Animals Incubation Dose-Response Relationship Drug Endocrine and Autonomic Systems beta-Endorphin Rats Inbred Strains In vitro Rats medicine.anatomical_structure Verapamil chemistry Potassium Endorphins medicine.drug |
| Zdroj: | Neuroendocrinology. 35:163-168 |
| ISSN: | 1423-0194 0028-3835 |
| DOI: | 10.1159/000123375 |
| Popis: | The effect of substance P (SP) on the release of beta-endorphin-like immunoreactivity (beta-EpLI) in rats was studied in vivo and in vitro. Intravenous injection of 5 micrograms/100 g BW of SP resulted in significant increase in the plasma beta-EpLI level after 15 and 30 min. SP at concentrations of 10(-9) to 10(-6) M also caused dose-dependent stimulation of beta-EpLI release from dispersed cells of rat anterior pituitary. On gel-chromatography, the beta-EpLI released by incubation of the cells with 10(-7) M SP separated into two components, eluted in the same positions as human beta-lipotropin and human beta-Ep, respectively. Release of beta-EpLI from the cells was increased by the addition of K+ at high concentration (53 mM) in a Ca++-dependent manner. Addition of 10(-3) M verapamil to the incubation medium inhibited SP-induced beta-EpLI release from the cells. Ouabain (10(-5) M) had a stimulatory effect on beta-EpLI release which was not additive with that of SP. These results indicate that SP acts directly on the anterior pituitary cells to stimulate beta-EpLI release and that calcium ion is involved in the mechanism of this effect. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|