| Autor: |
Tianyi Chen, George Dalton, Seh-Hoon Oh, Raquel Maeso-Diaz, Kuo Du, Rachel A. Meyers, Cynthia Guy, Manal F. Abdelmalek, Ricardo Henao, Paolo Guarnieri, Steven S. Pullen, Simon Gregory, Joseph Locker, J. Mark Brown, Anna Mae Diehl |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Cellular and molecular gastroenterology and hepatology. |
| ISSN: |
2352-345X |
| Popis: |
Nonalcoholic steatohepatitis (NASH), a leading cause of cirrhosis, strongly associates with the Metabolic Syndrome (MetS), an insulin-resistant pro-inflammatory state that disrupts energy balance and promotes progressive liver degeneration. We aimed to define the role of Smoothened (Smo), an obligatory component of Hedgehog signaling pathway, in controlling hepatocyte metabolic homeostasis and thereby, susceptibility to NASH.We conditionally deleted Smo in hepatocytes of healthy chow-fed mice and performed metabolic phenotyping, coupled with single-cell RNA sequencing, to characterize the role of hepatocyte Smo in regulating basal hepatic and systemic metabolic homeostasis. Liver RNA-seq datasets from two large human cohorts were also analyzed to define the relationship between Smo and NASH susceptibility in people.Hepatocyte Smo deletion inhibited the Hedgehog pathway and promoted fatty liver, hyperinsulinemia and insulin resistance. We identified a plausible mechanism whereby inactivation of Smo stimulated the mTORC1-SREBP1c signaling axis which promoted lipogenesis while inhibiting the hepatic insulin cascade. Transcriptomics of bulk- and single- Smo-deficient hepatocytes supported suppression of insulin signaling and also revealed molecular abnormalities associated with oxidative stress and mitochondrial dysfunction. Analysis of human bulk RNA-seq data revealed that Smo expression was i) highest in healthy livers, ii) lower in livers with NASH than in those with simple steatosis, iii) negatively correlated with markers of insulin resistance and liver injury, and iv) declined progressively as fibrosis severity worsened.Hedgehog pathway controls insulin sensitivity and energy homeostasis in adult livers. Loss of hepatocyte Hedgehog activity induces hepatic and systemic metabolic stress and enhances susceptibility to NASH by promoting hepatic lipoxicity and insulin resistance. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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