The Poly(C)-Binding Protein-1 Regulates Expression of the Androgen Receptor
| Autor: | Geoffrey Trew, Roberto Dina, Patricia Ellis, Hiroshi Kaneda, Kunal Shah, Mark Christian, Jan J. Brosens, Jenny Higham, Sadaf Ghaem-Maghami, Brianna Cloke, Luca Fusi, Stuart Lavery |
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| Rok vydání: | 2010 |
| Předmět: |
Adult
Male Ribonuclease III medicine.medical_specialty Adolescent Biology Heterogeneous-Nuclear Ribonucleoproteins DEAD-box RNA Helicases Endometrium Young Adult Endocrinology Internal medicine LNCaP Androgen Receptor Antagonists medicine Humans RNA Small Interfering Receptor Cells Cultured Regulation of gene expression Messenger RNA Gene knockdown Binding protein RNA-Binding Proteins Decidualization Cell Differentiation Molecular biology DNA-Binding Proteins Androgen receptor Gene Expression Regulation Receptors Androgen Gene Knockdown Techniques Female Stromal Cells |
| Zdroj: | Endocrinology. 151:3954-3964 |
| ISSN: | 1945-7170 0013-7227 |
| DOI: | 10.1210/en.2009-1264 |
| Popis: | The androgen receptor (AR) is a ligand-dependent transcription factor, expressed in male and female reproductive organs, and essential for normal reproduction in both sexes. The levels of AR are tightly controlled in androgen-responsive cells in which it plays a central role in the regulation of target gene expression. The AR is abundantly expressed in human endometrial stromal cells (HESCs), but levels decline markedly after differentiation into decidual cells in vivo and in primary cultures. Decidualization profoundly down-regulated AR protein levels with no discernible effect on either AR mRNA or protein stability, suggesting that loss of the receptor was a consequence of translational inhibition. Here we show that HESCs express three RNA-binding proteins, Hu antigen R and the poly(C)-binding proteins PCBP1 and PCBP2, that reportedly target the 3′-untranslated region of AR transcripts. Only PCBP1 expression was enhanced in secretory endometrium in vivo and in decidualizing HESCs. Furthermore, knockdown of PCBP1 in decidualizing cells was sufficient to restore AR protein levels, indicating that loss of the AR protein is primarily the consequence of a translational block. PCBP1 also blocked AR translation in a cell-free system, although this did not require binding to the 3′-untranslated region of the receptor mRNA. Furthermore, knockdown of PCBP1 in the prostate cancer LNCaP cell line also increased AR protein. Therefore, PCBP1 plays a major role in the dynamic expression of AR in both male and female androgen-responsive cells. |
| Databáze: | OpenAIRE |
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