The Scavenger Receptor Class B, Type I Is a Primary Determinant of Paraoxonase-1 Association With High-Density Lipoproteins
Autor: | Ursula Seidler, Marie-Claude Brulhart-Meynet, Anurag K. Singh, Theo J.C. Van Berkel, Richard W. James, Sara Deakin, Brigitte Riederer, Ruud Out |
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Rok vydání: | 2010 |
Předmět: |
medicine.medical_specialty
Antioxidants/metabolism Down-Regulation Gene Expression Antioxidants Lipoproteins HDL/*metabolism Cricetinae Internal medicine medicine Animals Humans Scavenger receptor Receptor Cells Cultured ddc:616 Gene knockdown biology Aryldialkylphosphatase Chinese hamster ovary cell Paraoxonase Scavenger Receptors Class B/genetics/*metabolism Transfection Scavenger Receptors Class B Aryldialkylphosphatase/blood/genetics/*metabolism PON1 Endocrinology biology.protein Lipoproteins HDL Cardiology and Cardiovascular Medicine Lipoprotein |
Zdroj: | Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 30, No 11 (2010) pp. 2121-2127 |
ISSN: | 1524-4636 1079-5642 |
DOI: | 10.1161/atvbaha.110.209122 |
Popis: | Objective— To examine the contribution of the scavenger receptor (SR) BI to the mechanism by which high-density lipoprotein (HDL) acquires paraoxonase-1 (PON1). Methods and Results— Serum PON1 activity contributes to the antioxidant capacity of HDLs and is suggested to be an independent risk factor for atherosclerosis. The association of PON1 with HDL is a major determinant of its serum activity levels. PON1 secretion was studied in stably transfected Chinese hamster ovary and HepG2 models. Complementary analyses were performed in transgenic models. Modulation of SR-BI expression, by SR-BI small and interfering RNA knockdown and pharmacologically, correlated with significant changes ( P Conclusion— The present study identifies SR-BI as a major determinant of the capacity of HDL to acquire PON1. It reinforces the concept of the receptor as a docking molecule, allowing communication between HDL and the cell, and extends the importance of SR-BI to HDL metabolism and function. |
Databáze: | OpenAIRE |
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