The Scavenger Receptor Class B, Type I Is a Primary Determinant of Paraoxonase-1 Association With High-Density Lipoproteins

Autor: Ursula Seidler, Marie-Claude Brulhart-Meynet, Anurag K. Singh, Theo J.C. Van Berkel, Richard W. James, Sara Deakin, Brigitte Riederer, Ruud Out
Rok vydání: 2010
Předmět:
Zdroj: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 30, No 11 (2010) pp. 2121-2127
ISSN: 1524-4636
1079-5642
DOI: 10.1161/atvbaha.110.209122
Popis: Objective— To examine the contribution of the scavenger receptor (SR) BI to the mechanism by which high-density lipoprotein (HDL) acquires paraoxonase-1 (PON1). Methods and Results— Serum PON1 activity contributes to the antioxidant capacity of HDLs and is suggested to be an independent risk factor for atherosclerosis. The association of PON1 with HDL is a major determinant of its serum activity levels. PON1 secretion was studied in stably transfected Chinese hamster ovary and HepG2 models. Complementary analyses were performed in transgenic models. Modulation of SR-BI expression, by SR-BI small and interfering RNA knockdown and pharmacologically, correlated with significant changes ( P Conclusion— The present study identifies SR-BI as a major determinant of the capacity of HDL to acquire PON1. It reinforces the concept of the receptor as a docking molecule, allowing communication between HDL and the cell, and extends the importance of SR-BI to HDL metabolism and function.
Databáze: OpenAIRE
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