Genome-wide analyses of gene expression during mouse endochondral ossification
| Autor: | Claudine G. James, Lee-Anne Stanton, Frank Beier, Veronica Ulici, Hanga Agoston, T. Michael Underhill |
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| Rok vydání: | 2009 |
| Předmět: |
Transcription
Genetic lcsh:Medicine Context (language use) Biology Chondrocyte Rheumatology/Cartilage Biology and Osteoarthritis 03 medical and health sciences Mice Developmental Biology/Molecular Development 0302 clinical medicine Chondrocytes medicine Animals Growth Plate lcsh:Science Endochondral ossification 030304 developmental biology DNA Primers Regulation of gene expression Genetics 0303 health sciences Multidisciplinary Bone Development Genome Base Sequence Ossification Gene Expression Profiling lcsh:R Gene Expression Regulation Developmental Genetics and Genomics/Gene Expression Genetics and Genomics/Bioinformatics Chondrogenesis Cell biology Gene expression profiling medicine.anatomical_structure Developmental Biology/Cell Differentiation lcsh:Q medicine.symptom DNA microarray 030217 neurology & neurosurgery Research Article |
| Zdroj: | PLoS ONE Paediatrics Publications PLoS ONE, Vol 5, Iss 1, p e8693 (2010) |
| ISSN: | 1932-6203 |
| Popis: | Background: Endochondral ossification is a complex process involving a series of events that are initiated by the establishment of a chondrogenic template and culminate in its replacement through the coordinated activity of osteoblasts, osteoclasts and endothelial cells. Comprehensive analyses of in vivo gene expression profiles during these processes are essential to obtain a complete understanding of the regulatory mechanisms involved. Methodology/Principal Findings: To address these issues, we completed a microarray screen of three zones derived from manually segmented embryonic mouse tibiae. Classification of genes differentially expressed between each respective zone, functional categorization as well as characterization of gene expression patterns, cytogenetic loci, signaling pathways and functional motifs both confirmed reported data and provided novel insights into endochondral ossification. Parallel comparisons of the microdissected tibiae data set with our previously completed micromass culture screen further corroborated the suitability of micromass cultures for modeling gene expression in chondrocyte development. The micromass culture system demonstrated striking similarities to the in vivo microdissected tibiae screen; however, the micromass system was unable to accurately distinguish gene expression differences in the hypertrophic and mineralized zones of the tibia. Conclusions/Significance: These studies allow us to better understand gene expression patterns in the growth plate and endochondral bones and provide an important technical resource for comparison of gene expression in diseased or experimentally-manipulated cartilages. Ultimately, this work will help to define the genomic context in which genes are expressed in long bones and to understand physiological and pathological ossification. © 2010 James et al. |
| Databáze: | OpenAIRE |
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