Dynamic expression of leukocyte innate immune genes in whole blood from horses with lipopolysaccharide-induced acute systemic inflammation
| Autor: | Pia Haubro Andersen, Anne Mette L. Vinther, Kerstin Skovgaard, Peter M. H. Heegaard |
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| Rok vydání: | 2014 |
| Předmět: |
Lipopolysaccharides
Male Lipopolysaccharide Systemic inflammation NEONATAL FOALS chemistry.chemical_compound Leukocytes Innate immunity Cross-Over Studies INFUSION Baseline expression variability General Medicine Cytokines Female VETERINARY medicine.symptom Research Article Inflammation Biology Sepsis ORGAN DYSFUNCTION Immunity medicine Animals Horses HEALTHY Gene expression dynamics MONONUCLEAR-CELLS Innate immune system General Veterinary Septic shock Equine SEPTIC SHOCK PROFILES medicine.disease veterinary(all) Immunity Innate Endotoxemia Systemic inflammatory response syndrome SEVERE SEPSIS chemistry Gene Expression Regulation Immunology Horse Diseases Time course study ENDOTOXIN CHALLENGE EQUINE BLOOD Transcriptome |
| Zdroj: | BMC Veterinary Research Vinther, A M L, Skovgaard, K, Heegaard, P M H & Andersen, P H 2015, ' Dynamic expression of leukocyte innate immune genes in whole blood from horses with lipopolysaccharide-induced acute systemic inflammation ', B M C Veterinary Research, vol. 11 . https://doi.org/10.1186/s12917-015-0450-5 Vinther, A M L, Skovgaard, K, Heegaard, P M H & Andersen, P H 2015, ' Dynamic expression of leukocyte innate immune genes in whole blood from horses with lipopolysaccharide-induced acute systemic inflammation ', B M C Veterinary Research, vol. 11, 134 . https://doi.org/10.1186/s12917-015-0450-5 |
| ISSN: | 1746-6148 |
| DOI: | 10.1186/s12917-015-0450-5 |
| Popis: | Background In horses, insights into the innate immune processes in acute systemic inflammation are limited even though these processes may be highly important for future diagnostic and therapeutic advances in high-mortality disease conditions as the systemic inflammatory response syndrome (SIRS) and sepsis. Therefore, the aim of this study was to investigate the expression of 31 selected blood leukocyte immune genes in an equine model of acute systemic inflammation to identify significantly regulated genes and to describe their expression dynamics during a 24-h experimental period. Systemic inflammation was induced in 6 adult horses by the intravenous injection of 1 μg lipopolysaccharide (LPS) per kg btw. Sixteen blood samples were collected for each horse at predetermined intervals and analyzed by reverse transcription quantitative real-time PCR. Post-induction expression levels for each gene were compared with baseline levels. Results Systemic inflammation was confirmed by the presence of clinical and hematological changes which were consistent with SIRS. The clinical response to LPS was transient and brief as all horses except one showed unaltered general demeanor after 24 h. Twenty-two leukocyte genes were significantly regulated at at least one time point during the experimental period. By close inspection of the temporal responses the dynamic changes in mRNA abundance revealed a very rapid onset of both pro- and anti-inflammatory mediators and a substantial variation in both expression magnitudes and duration of changes between genes. A majority of the 22 significantly regulated genes peaked within the first 8 h after induction, and an on-going, albeit tightly controlled, regulation was seen after 24 h despite approximate clinical recovery. Conclusions This first broad study of gene expressions in blood leukocytes during equine acute LPS-induced systemic inflammation thoroughly characterized a highly regulated and dynamic innate immune response. These results provide new insights into the molecular mechanisms of equine systemic inflammation. Electronic supplementary material The online version of this article (doi:10.1186/s12917-015-0450-5) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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