Dependence of Endocochlear Potential on Basolateral Na+ and Cl- Concentration: A Study Using Vascular and Perilymph Perfusion
| Autor: | Susumu Shida, Manabu Miyamoto, Noboru Abe, Yasushi Murakami, Yusuke Imai, Masahiko Shindo |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Pathology
medicine.medical_specialty Physiology Endocochlear potential Potassium Sodium Guinea Pigs Biological Transport Active chemistry.chemical_element Perilymph Basement Membrane Guinea pig Chlorides medicine Animals Furosemide General Medicine Cochlea Perfusion chemistry Symporter Cochlear Microphonic Potentials Biophysics Female Peptides medicine.drug |
| Zdroj: | The Japanese Journal of Physiology. 42:617-630 |
| ISSN: | 0021-521X |
| DOI: | 10.2170/jjphysiol.42.617 |
| Popis: | Inside-positive endocochlear potential (EP) and high potassium concentration in the endocochlear duct are generated by transepithelial K+ transport in marginal cells of the stria vascularis. In order to estimate the degree of involvement of Na+ and Cl- in K+ transport in marginal cells, EP in guinea pigs was measured under artificial vascular and perilymphatic perfusion in situ. Na+ depletion due to both vascular and perilymphatic perfusion decreased EP by -10.0 +/- 4.1 mV (delta EP = -86 +/- 5.2 mV, n = 5) from the control value of 78 +/- 4.3 mV (p < 0.01). Cl- depletion due to vascular and perilymphatic perfusion also decreased EP by -10.0 +/- 4.9 mV (delta EP = 8.5 +/- 4.8 mV, n = 6) from the control value of 77 +/- 5.1 mV (p < 0.01). However, under either vascular or perilymphatic perfusion, even lowering of Na+ or Cl- concentration in the perfusate decreased EP only slightly compared to the results under both vascular and perilymphatic perfusion. Furosemide, a blocker of Na+/K+/2Cl- symport, decreased EP under vascular perfusion. This dependency of EP on basolateral Na+ and Cl- concentration strongly suggests that K+ transport by the marginal cell is dependent on the basolateral Na+ and Cl- concentration, and that Na+/K+/2Cl- symport is raised as a possible mechanism for Na+ and Cl- dependency of EP. |
| Databáze: | OpenAIRE |
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