Structural characterization of an unprecedented lectin-like antitumoral anti-MUC1 antibody

Autor: Ismael Compañón, Filipa Marcelo, Vincenzo Mangini, Ana García-García, Francisco Corzana, Alicia Asín, Javier Macías-León, Inês S. Albuquerque, Iris A. Bermejo, Hiren J. Joshi, Gonçalo J. L. Bernardes, Ramon Hurtado-Guerrero, Juan Luis Asensio, Ola Blixt, Ester Jiménez-Moreno, Helena Coelho, Roberto Fiammengo
Přispěvatelé: Ministerio de Ciencia, Innovación y Universidades (España), Ministerio de Economía y Competitividad (España), Danish National Research Foundation, Universidad de La Rioja, Lopes Bernardes, Goncalo [0000-0001-6594-8917], Apollo - University of Cambridge Repository
Rok vydání: 2020
Předmět:
Glycosylation
Protein Conformation
Antineoplastic Agents
Peptide
Molecular Dynamics Simulation
010402 general chemistry
Cancer Vaccines
01 natural sciences
Catalysis
Epitope
Structure-Activity Relationship
03 medical and health sciences
chemistry.chemical_compound
Protein structure
Lectins
Materials Chemistry
antibodies
Humans
Structure–activity relationship
030304 developmental biology
chemistry.chemical_classification
0303 health sciences
glycopeptides
mucin-1
antibodies
biology
Chemistry
Mucin-1
Glycopeptides
Metals and Alloys
Antibodies
Monoclonal
Lectin
Hydrogen Bonding
General Chemistry
Peptide Fragments
Glycopeptide
0104 chemical sciences
Surfaces
Coatings and Films
Electronic
Optical and Magnetic Materials
Biochemistry
Ceramics and Composites
biology.protein
Drug Screening Assays
Antitumor
Antibody
Zdroj: Digital.CSIC. Repositorio Institucional del CSIC
instname
Macías-León, J, Bermejo, I A, Asín, A, García-García, A, Compañón, I, Jiménez-Moreno, E, Coelho, H, Mangini, V, Albuquerque, I S, Marcelo, F, Asensio, J L, Bernardes, G J L, Joshi, H J, Fiammengo, R, Blixt, O, Hurtado-Guerrero, R & Corzana, F 2020, ' Structural characterization of an unprecedented lectin-like antitumoral anti-MUC1 antibody ', Chemical communications (Cambridge, England), vol. 56, no. 96, pp. 15137-15140 . https://doi.org/10.1039/d0cc06349e
Zaguán. Repositorio Digital de la Universidad de Zaragoza
Popis: The molecular basis of antibody 5E5, which recognizes the entire GalNAc unit as a primary epitope is disclosed. The antibody's contacts with the peptide are mostly limited to two residues, allowing it to show some degree of promiscuity. These findings open the door to the chemical design of peptide-mimetics for developing efficient anti-cancer vaccines and diagnostic tools. This journal is
Funding from the Agencia Estatal Investigacion of Spain (AEI,RTI-2018-099592-B-C21, CTQ2013-44367-C2-2-P, BFU2016-75633-P,PID2019-105451GB-I00 and PID2019-107476GB-I00), ARAID, the Italian Ministry of Education, University and Research (PRIN2015 contract nr. 2015RNWJAM), the Royal Society (URF\R\180019),the Danish National Research Foundation (DNRF107) and FCTPortugal (iFCT, IF/00624/2015, and Doctoral Studentship SFRH/BD/111556/2015, IF/00780/2015; PTDC/BIA-MIB/31028/2017, UCIBIOUIDB/04378/2020 and NMR Infrastructure project 22161) isacknowledged. I. A. B. and A. A. thank the AECC for predoctoralfellowships. E. J.-M. thanks Universidad de La Rioja for a post-doctoral fellowship. We thank H. Clausen and U. Mandel forproviding us the complete 5E5 antibody.
Databáze: OpenAIRE
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