Gamete expression of TALE class HD genes activates the diploid sporophyte program in Marchantia polymorpha
| Autor: | Sabine Zachgo, Madlen I Rast-Somssich, Tom Dierschke, John L. Bowman, Eduardo Flores-Sandoval, Felix Althoff |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
QH301-705.5 Science plant evolution Biology 01 natural sciences General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Marchantia polymorpha Marchantia medicine TALE-homeodomain Biology (General) Gene 030304 developmental biology Genetics 0303 health sciences Zygote General Immunology and Microbiology General Neuroscience fungi alternation of generations Sporophyte General Medicine biology.organism_classification medicine.anatomical_structure Alternation of generations Medicine Gamete Ploidy 010606 plant biology & botany |
| Zdroj: | eLife, Vol 10 (2021) |
| ISSN: | 2050-084X |
| Popis: | Eukaryotic life cycles alternate between haploid and diploid phases and in phylogenetically diverse unicellular eukaryotes, expression of paralogous homeodomain genes in gametes primes the haploid-to-diploid transition. In the unicellular chlorophyte alga Chlamydomonas, KNOX and BELL TALE-homeodomain genes mediate this transition. We demonstrate that in the liverwort Marchantia polymorpha, paternal (sperm) expression of three of five phylogenetically diverse BELL genes, MpBELL234, and maternal (egg) expression of both MpKNOX1 and MpBELL34 mediate the haploid-to-diploid transition. Loss-of-function alleles of MpKNOX1 result in zygotic arrest, whereas a loss of either maternal or paternal MpBELL234 results in variable zygotic and early embryonic arrest. Expression of MpKNOX1 and MpBELL34 during diploid sporophyte development is consistent with a later role for these genes in patterning the sporophyte. These results indicate that the ancestral mechanism to activate diploid gene expression was retained in early diverging land plants and subsequently co-opted during evolution of the diploid sporophyte body. |
| Databáze: | OpenAIRE |
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