Microchip-based enzyme-linked immunosorbent assay (microELISA) system with thermal lens detection
Autor: | Maho Yamanaka, Hiroko Kimura, Takehiko Kitamori, Tomokazu Hagino, Manabu Tokeshi, Kiichi Sato |
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Rok vydání: | 2004 |
Předmět: |
Spectrum analyzer
Microscope Biomedical Engineering Analytical chemistry Liquid phase Enzyme-Linked Immunosorbent Assay Bioengineering Biochemistry law.invention Interferon-gamma law Lab-On-A-Chip Devices Microchip Analytical Procedures medicine Humans Lenses Miniaturization Microchannel Chromatography medicine.diagnostic_test Chemistry Substrate (chemistry) Equipment Design General Chemistry Equipment Failure Analysis Systems Integration Lens (optics) Thermography Immunoassay Flow Injection Analysis |
Zdroj: | Lab on a Chip. 4:570 |
ISSN: | 1473-0189 1473-0197 |
DOI: | 10.1039/b411356j |
Popis: | A microchip-based enzyme-linked immunosorbent assay (microELISA) system was developed and interferon-gamma was successfully determined. The system was composed of a microchip with a Y-shaped microchannel and a dam structure, polystyrene microbeads, and a thermal lens microscope (TLM). All reactions required for the immunoassay were done in the microchannel by successive introduction of a sample and regents. The enzyme reaction product, in a liquid phase, was detected downstream in the channel using the TLM as substrate solution was injected. The antigen-antibody reaction time was shortened by the microchip integration. The limit of the determination was improved by adopting the enzyme label. Moreover, detection procedures were greatly simplified and required time for the detection was significantly cut. The system has good potential to be developed as a small and automated high throughput analyzer. |
Databáze: | OpenAIRE |
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