Ectopic Expression of a Bacterium NhaD-type Na+/H+ Antiporter Leads to Increased Tolerance to Combined Salt/Alkali Stresses
| Autor: | Fang Wang, Xiaomin Wu, Yan-He Ma, Li-Li Wang, Nai-Qin Zhong, Gui-Xian Xia, Li-Bo Han |
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| Rok vydání: | 2012 |
| Předmět: |
Sodium-Hydrogen Exchangers
Chemistry Transgene Antiporter Sodium Salt Tolerance Plant Science Vacuole Hydrogen-Ion Concentration Plants Genetically Modified Plant cell Subcellular localization Biochemistry Molecular biology General Biochemistry Genetics and Molecular Biology Cell Line Cytosol Tobacco Vacuoles Botany Alkaliphile Extremophile Ectopic expression Gammaproteobacteria |
| Zdroj: | Journal of Integrative Plant Biology. 54:412-421 |
| ISSN: | 1672-9072 |
| DOI: | 10.1111/j.1744-7909.2012.01129.x |
| Popis: | AaNhaD, a gene isolated from the soda lake alkaliphile Alkalimonas amylolytica, encodes a Na(+) /H(+) antiporter crucial for the bacterium's resistance to salt/alkali stresses. However, it remains unknown whether this type of bacterial gene may be able to increase the tolerance of flowering plants to salt/alkali stresses. To investigate the use of extremophile genetic resources in higher plants, transgenic tobacco BY-2 cells and plants harboring AaNhaD were generated and their stress tolerance was evaluated. Ectopic expression of AaNhaD enhanced the salt tolerance of the transgenic BY-2 cells in a pH-dependent manner. Compared to wild-type controls, the transgenic cells exhibited increased Na(+) concentrations and pH levels in the vacuoles. Subcellular localization analysis indicated that AaNhaD-GFP fusion proteins were primarily localized in the tonoplasts. Similar to the transgenic BY-2 cells, AaNhaD-overexpressing tobacco plants displayed enhanced stress tolerance when grown in saline-alkali soil. These results indicate that AaNhaD functions as a pH-dependent tonoplast Na(+) /H(+) antiporter in plant cells, thus presenting a new avenue for the genetic improvement of salinity/alkalinity tolerance. |
| Databáze: | OpenAIRE |
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