MiR-495 regulates proliferation and migration in NSCLC by targeting MTA3

Autor: Ping Li, Yuanyuan Wang, Xudong Chen, Wenqiao Zang, Guojun Zhang, Jingxia Chang, Huaqi Wang, Yuwen Du, Juan Li, Guoqiang Zhao, Heying Chu
Rok vydání: 2013
Předmět:
Zdroj: Tumor Biology. 35:3487-3494
ISSN: 1423-0380
1010-4283
Popis: Our previous studies have showed that metastasis-associated protein 3 (MTA 3) is overexpressed in non-small cell lung cancer (NSCLC) tissue, and increased MTA3 mRNA levels is a risk factor of lymph node metastasis. Using bioinformatics analyses, we found that MTA3 was a potential target of miR-495. However, the pathophysiological role of miR-495 and its relevance to the growth and development of NSCLC have yet to be investigated. The purpose of this study was to elucidate the molecular mechanisms by which miR-495 acts as a tumor suppressor in NSCLC. qRT-PCR data showed significant downregulation of miR-495 in 56 NSCLC tissue samples and 5 lung cancer cell lines, compared with their adjacent normal tissue; furthermore, western blotting analysis revealed MTA3 protein was overexpressed in the tumor samples compared with the matched adjacent normal tissue. MiR-495 was shown to not only inhibit the proliferation of lung cancer cells (A549 and Calu-3) but also to inhibit cell migration in vitro. Using western blotting and luciferase assays, MTA3 was identified as a target of miR-495. These findings suggest the importance of miR-495 targeting of MTA3 in the regulation of lung cancer growth and migration.
Databáze: OpenAIRE
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