Intervention effect of gamma aminobutyric acid on anxiety behavior induced by phthalate (2-ethylhexyl ester) in rats
| Autor: | Shuqin Liang, Huan Liu, Minhao Huang, Tongwang Yang, Youting Guo, Chunhong Liu, Zhicheng Fan |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
GABA Agents Prefrontal Cortex Intervention effect Environmental pollution 010501 environmental sciences Pharmacology Anxiety Nitric Oxide 01 natural sciences gamma-Aminobutyric acid Nitric oxide Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound Human health Random Allocation Diethylhexyl Phthalate medicine Animals gamma-Aminobutyric Acid 0105 earth and related environmental sciences biology Behavior Animal General Neuroscience Phthalate General Medicine Rats Nitric oxide synthase 030104 developmental biology chemistry biology.protein medicine.symptom Nitric Oxide Synthase medicine.drug |
| Zdroj: | The International journal of neuroscience. 128(10) |
| ISSN: | 1563-5279 |
| Popis: | Di(2-ethylhexyl) phthalate (DEHP) is one of the most widely used phthalate esters. The application of DEHP has caused serious environmental pollution and posed a threat to human health.A total of 30 male Sprague-Dawley rats were randomly divided into control group, DEHP group (500 mg/kg DEHP), low GABA (Gama-aminobutyric acid) group (500 mg/kg DEHP and 1 mg/kg GABA), medium GABA group (500 mg/kg DEHP and 2 mg/kg GABA) and high GABA group (500 mg/kg DEHP and 4 mg/kg GABA). The interventions continued for 30 consecutive days. Open-field test and elevated plus-maze test were used to detect behavioral changes of rats before and after interventions.The levels of nitric oxide and nitric oxide synthase in prefrontal cortex of rats were measured using enzyme-linked immunosorbent assay. DEHP and GABA treatment had no significant effects on the body weight of rats. GABA restored food utilization rate of rats impaired by DEHP to the level of healthy rats. According to open-field test and elevated plus-maze test, GABA alleviated the effects of DEHP on rat behaviors. Enzyme-linked immunosorbent assay showed that GABA was effective in reducing the levels of nitric oxide and nitric oxide synthase in rats treated with DEHP.DEHP exposure induced anxiety in rats, which may be achieved through elevating nitric oxide and nitric oxide synthase levels in prefrontal cortex of rats. However, the effects caused by DEHP could be alleviated by GABA. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|