Hand-Held Microanalytical Instrument for Chip-Based Electrophoretic Separations of Proteins

Autor: Jay A. A. West, Boyd Wiedenman, Julia A. Fruetel, Scott M. Ferko, Daniel Yee, Victoria A. VanderNoot, Ronald F. Renzi, Brent A. Horn, James F. Stamps, Robert W. Crocker
Rok vydání: 2004
Předmět:
Zdroj: Analytical Chemistry. 77:435-441
ISSN: 1520-6882
0003-2700
DOI: 10.1021/ac049214f
Popis: The design, fabrication, and demonstration of a hand-held microchip-based analytical instrument for detection and identification of proteins and other biomolecules are reported. The overall system, referred to as muChemLab, has a modular design that provides for reliability and flexibility and that facilitates rapid assembly, fluid and microchip replacement, troubleshooting, and sample analysis. Components include two independent separation modules that incorporate interchangeable fluid cartridges, a 2-cm-square fused-silica microfluidic chip, and a miniature laser-induced fluorescence detection module. A custom O-ring sealed manifold plate connects chip access ports to a fluids cartridge and a syringe injection port and provides sample introduction and world-to-chip interface. Other novel microfluidic connectors include capillary needle fittings for fluidic connection between septum-sealed fluid reservoirs and the manifold housing the chip, enabling rapid chip priming and fluids replacement. Programmable high-voltage power supplies provide bidirectional currents up to 100 microAlpha at 5000 V, enabling real-time current and voltage monitoring and facilitating troubleshooting and methods development. Laser-induced fluorescence detection allows picomolar (10(-11) M) detection sensitivity of fluorescent dyes and nanomolar sensitivity (10(-9) M) for fluorescamine-labeled proteins. Migration time reproducibility was significantly improved when separations were performed under constant current control (0.5-1%) as compared to constant voltage control (2-8%).
Databáze: OpenAIRE
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