Nuclear maturation kinetics and in vitro fertilization of immature bovine oocytes injected into pre-ovulatory follicles
| Autor: | Nadja Gomes Alves, Margot Alves Nunes Dode, E. A. Lima, F. M. C. Caixeta, José Camisão de Souza, C. C. R. Quintão, R. E. Orlandi, José Nelio de Sousa Sales, A. P. C. Santos, Luiz Sérgio de Almeida Camargo, L. M. S. Simoes, U. R. Fernandes, S. A. D. Rodrigues, M. P. Bottino |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_treatment
Kinetics Fertilization in Vitro Andrology 03 medical and health sciences Follicle Oogenesis 0302 clinical medicine Human fertilization Ovarian Follicle Food Animals Recovery rate medicine Animals Small Animals 030219 obstetrics & reproductive medicine In vitro fertilisation Zygote Equine Chemistry 0402 animal and dairy science Embryo 04 agricultural and veterinary sciences Oocyte 040201 dairy & animal science medicine.anatomical_structure Oocytes Cattle Female Animal Science and Zoology |
| Zdroj: | Theriogenology. 166:90-96 |
| ISSN: | 0093-691X |
| Popis: | The maturation kinetics and in vitro fertilization of immature bovine oocytes injected by the intra-follicular oocyte injection (IFOT) technique into pre-ovulatory follicles of previously synchronized cows were evaluated. In Experiment 1, grade I, II and III cumulus-oocyte complexes (COCs) were randomly distributed to one of three Groups: Matvitro22 (COCs matured in vitro for 22 h), MatFol20 and MatFol28 (COCs matured in vivo after being injected into a pre-ovulatory follicle of previously synchronized cows for 19.8 ± 0.1 h and 28.3 ± 0.1 h, respectively). Cows received 12.5 mg of LH (Lutropin, Bioniche, Canada) at the time of IFOT in the MatFol20 Group or 10 h after IFOT in the MatFol28 Group. MatFol20 and MatFol28 COCs were aspirated approximately 20 h after the LH injection for nuclear maturation kinetics and recovery rate assessment. In Experiment 2, grade I, II, and III COCs were randomly distributed into two Groups: Matvitro22 Group, COCs were matured and fertilized in vitro, and MatFol20 Group, COCs were matured as in the MatFol20 Group in Experiment 1, but COCs were fertilized in vitro. Putative zygotes were classified as fertilized, unfertilized or polyspermic. In Experiment 1, the recovery rate was lower (P 0.001) in the MatFol20 Group (52.9%, 91/172) compared with MatFol28 (72.9%, 113/155). Rate of oocytes in germinal vesicle stage, metaphase I, anaphase I and telophase I were similar among Groups. However, oocytes matured in vivo for 28.3 h had lower rate of metaphase II (P = 0.001) and greater rates of degenerated (P = 0.001) and parthenogenetically activated (P = 0.001) oocytes. In experiment 2, the rates of polyspermy and degenerated were similar between Groups. However, the rate of fertilized oocytes was greater (P = 0.05) in oocytes in the MatFol20 Group. It is concluded that oocyte in vivo maturation for 19.8 h after IFOT does not compromise the nuclear maturation kinetics and increases in vitro fertilization rates. However, the extra 10 h of intra-follicular incubation time decreased oocyte viability. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect