An experimental model of contact dermatitis: Evaluation of the oxidative profile of Wistar rats treated with free and nanoencapsulated clobetasol

Autor:	Viviane do Carmo Gonçalves Souza, M C Fontana, Nara Maria Beck Martins, Claudia de Mello Bertoncheli, Ruy Carlos Ruver Beck, Tatiana Montagner Dalcin Bertoldo, Karine Bizzi Schlemmer, Jeandre Augusto dos Santos Jaques, João Felipe Peres Rezer, Jader B. Ruchel, Kelly de Vargas Pinheiro, Daniela B.R. Leal, Josiane Bizzi Schlemmer
Rok vydání:	2012
Předmět:	Antioxidant Physiology Thiobarbituric acid medicine.medical_treatment Clinical Biochemistry Pharmacology Dermatitis Contact medicine.disease_cause Biochemistry Vaseline chemistry.chemical_compound medicine TBARS Animals Rats Wistar Research Articles Clobetasol Drug Carriers biology Superoxide Dismutase Chemistry Biochemistry (medical) Cell Biology Catalase Nanostructures Rats Oxidative Stress NPSH biology.protein Female Lipid Peroxidation Clobetasol propionate Oxidative stress medicine.drug
Zdroj:	Redox Report. 17:206-213
ISSN:	1743-2928 1351-0002
Popis:	Objective An experimental animal model of contact dermatitis (CD) was used to investigate the effects of free and nanoencapsulated clobetasol propionate on the skin and on the oxidative profile of liver tissue. Methods Female Wistar rats were divided into six groups, each containing eight rats. The first group, control (C), was sensitized with solid vaseline. Group 2, (CD), was sensitized with 5% NiSO(4). Groups 3 and 4 were sensitized with 5% NiSO(4) and treated with free (FC) and nanoencapsulated (NC) clobetasol (0.42 mg/g), respectively, daily for 5 days. Group 5 was treated with nanoencapsulated clobetasol (0.42 mg/g) on days 1, 3, and 5 (C135) and group 6 received a hydrogel containing empty nanoparticles (NP) daily for 5 days. Thiobarbituric acid reactive substances (TBARS), carbonyl levels, non-protein sulfhydryl groups (NPSH) and catalase activity were measured in liver homogenates. Results A significant increase was observed in the levels of TBARS, NPSH, and catalase activity for the groups CD and NP. Discussion Our results suggest that both NiSO(4) sensitization and NP administration induced oxidation of cellular lipids and activated the antioxidant enzyme catalase to protect from this damage. These results also indicated that daily treatment with the free and nanoencapsulated clobetasol, as well as treatment with the nanoencapsulated clobetasol every other day, were able to prevent these redox alterations and protect against histological damage.
Databáze:	OpenAIRE
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