Experimental repair of phrenic nerve using a polyglycolic acid and collagen tube
| Autor: | Hiroyuki Tao, Tatsuo Nakamura, Shigeyuki Morino, Seijun Fukuda, Katsuaki Endo, Akira Nakada, Shin-ichi Itoi, Yuji Inada, Makoto Yoshitani |
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| Rok vydání: | 2007 |
| Předmět: |
Thorax
Pulmonary and Respiratory Medicine medicine.medical_specialty Group ii Diaphragmatic breathing Sensitivity and Specificity Beagle Neurosurgical Procedures Dogs Implants Experimental Animals Medicine Tube (fluid conveyance) Phrenic nerve business.industry Biopsy Needle Anatomy Immunohistochemistry Nerve Regeneration Diaphragm (structural system) Surgery Electrophysiology Phrenic Nerve Disease Models Animal Breathing Collagen business Cardiology and Cardiovascular Medicine Polyglycolic Acid |
| Zdroj: | The Journal of Thoracic and Cardiovascular Surgery. 133(3):726-732.e3 |
| ISSN: | 0022-5223 |
| DOI: | 10.1016/j.jtcvs.2006.08.089 |
| Popis: | Objective The feasibility of a nerve guide tube for regeneration of the phrenic nerve with the aim of restoring diaphragmatic function was evaluated in a canine model. Methods The nerve tube, made of woven polyglycolic acid mesh, had a diameter of 3 mm and was filled with collagen sponge. This polyglycolic acid–collagen tube was implanted into a 10-mm gap created by transection of the right phrenic nerve in 9 beagle dogs. The tubes were implanted without a tissue covering in 5 of the 9 dogs (group I), and the tubes were covered with a pedicled pericardial fat pad in 4 dogs (group II). Chest x-ray films, muscle action potentials, and histologic samples were examined 4 to 12 months after implantation. Results All of the dogs survived without any complications. X-ray film examination showed that the right diaphragm was paralyzed and elevated in all dogs until 3 months after implantation. At 4 months, movement of the diaphragm in the implanted side was observed during spontaneous breathing in 1 dog of group I and in 3 dogs of group II. In the dogs showing diaphragm movement, muscle action potentials were evoked in the diaphragm muscle, indicating restoration of nerve function. Regeneration of the phrenic nerve structure was also examined on the reconstructed site using electron microscopy. Conclusion The polyglycolic acid–collagen tube induced functional recovery of the injured phrenic nerve and was aided by coverage with a pedicled pericardial fat pad. |
| Databáze: | OpenAIRE |
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