Characterization of HCN and cardiac function in a colonial ascidian

Autor: Stefano Tiozzo, Jungho Ohn, Michael Liebling, Anthony W. De Tomaso, Annette Hellbach
Přispěvatelé: Laboratoire de Biologie du Développement de Villefranche sur mer (LBDV), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de la Mer de Villefranche (IMEV), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Cardiac function curve
Agonist
Heartbeat
Physiology
medicine.drug_class
Adrenergic beta-Antagonists
Cyclic Nucleotide-Gated Cation Channels
Botryllus schlosseri
03 medical and health sciences
0302 clinical medicine
Piperidines
Biological Clocks
Receptors
Adrenergic
beta

Genetics
medicine
Animals
Humans
Urochordata
Molecular Biology
[SDV.BDD]Life Sciences [q-bio]/Development Biology
Ecology
Evolution
Behavior and Systematics

ComputingMilieux_MISCELLANEOUS
030304 developmental biology
Metoprolol
Fibrillation
0303 health sciences
Sequence Homology
Amino Acid

biology
Tubular heart
Gene Expression Regulation
Developmental

Heart
Anatomy
Benzazepines
biology.organism_classification
3. Good health
Cardiovascular physiology
Cell biology
cardiovascular system
RNA
Animal Science and Zoology
medicine.symptom
Blood Flow Velocity
030217 neurology & neurosurgery
medicine.drug
Zdroj: Journal of Experimental Zoology Part A: Ecological Genetics and Physiology
Journal of Experimental Zoology Part A: Ecological Genetics and Physiology, Wiley, 2011, 315A (8), pp.476-486. ⟨10.1002/jez.695⟩
ISSN: 1932-5223
1932-5231
DOI: 10.1002/jez.695⟩
Popis: Hyperpolarization-activated, cyclic nucleotide-gated (HCN) channels generate the rhythmic beating of mammalian hearts. We identified an HCN homolog in the colonial ascidian Botryllus schlosseri, a nonvertebrate chordate which possesses a tubular heart that beats bidirectionally. Contractions initiate at one end of the heart and travel across the length of the organ, and these periodically reverse, suggesting the presence of two pacemakers, one on each side. We find that HCN expression is highly enriched in cells scattered throughout the myocardium. We functionally analyzed the role of HCN channels in heartbeat using the antagonists Cilobradine and Zatebradine, which decreased the heartbeat in a reversible manner. We also assessed the role of β-adrenoreceptors in regulating HCN function using the antagonist Metoprolol, which lowered heartbeat rate (HR), as well as the agonist Isoproterenol, which did not alter HR, but caused simultaneous beating, analogous to a fibrillation. Measurements of direction and velocity of blood flow by making use of a novel system to study heart function in model systems amenable to live imaging revealed a significant correlation between heartbeat arrhythmia and drug treatment, similar to that observed with the same drugs in vertebrates. These results suggest that the heart pacemaker in tunicates may be homologous to that in their vertebrate counterparts in both development and function.
Databáze: OpenAIRE