Characterization of HCN and cardiac function in a colonial ascidian
Autor: | Stefano Tiozzo, Jungho Ohn, Michael Liebling, Anthony W. De Tomaso, Annette Hellbach |
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Přispěvatelé: | Laboratoire de Biologie du Développement de Villefranche sur mer (LBDV), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de la Mer de Villefranche (IMEV), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) |
Jazyk: | angličtina |
Rok vydání: | 2011 |
Předmět: |
Cardiac function curve
Agonist Heartbeat Physiology medicine.drug_class Adrenergic beta-Antagonists Cyclic Nucleotide-Gated Cation Channels Botryllus schlosseri 03 medical and health sciences 0302 clinical medicine Piperidines Biological Clocks Receptors Adrenergic beta Genetics medicine Animals Humans Urochordata Molecular Biology [SDV.BDD]Life Sciences [q-bio]/Development Biology Ecology Evolution Behavior and Systematics ComputingMilieux_MISCELLANEOUS 030304 developmental biology Metoprolol Fibrillation 0303 health sciences Sequence Homology Amino Acid biology Tubular heart Gene Expression Regulation Developmental Heart Anatomy Benzazepines biology.organism_classification 3. Good health Cardiovascular physiology Cell biology cardiovascular system RNA Animal Science and Zoology medicine.symptom Blood Flow Velocity 030217 neurology & neurosurgery medicine.drug |
Zdroj: | Journal of Experimental Zoology Part A: Ecological Genetics and Physiology Journal of Experimental Zoology Part A: Ecological Genetics and Physiology, Wiley, 2011, 315A (8), pp.476-486. ⟨10.1002/jez.695⟩ |
ISSN: | 1932-5223 1932-5231 |
DOI: | 10.1002/jez.695⟩ |
Popis: | Hyperpolarization-activated, cyclic nucleotide-gated (HCN) channels generate the rhythmic beating of mammalian hearts. We identified an HCN homolog in the colonial ascidian Botryllus schlosseri, a nonvertebrate chordate which possesses a tubular heart that beats bidirectionally. Contractions initiate at one end of the heart and travel across the length of the organ, and these periodically reverse, suggesting the presence of two pacemakers, one on each side. We find that HCN expression is highly enriched in cells scattered throughout the myocardium. We functionally analyzed the role of HCN channels in heartbeat using the antagonists Cilobradine and Zatebradine, which decreased the heartbeat in a reversible manner. We also assessed the role of β-adrenoreceptors in regulating HCN function using the antagonist Metoprolol, which lowered heartbeat rate (HR), as well as the agonist Isoproterenol, which did not alter HR, but caused simultaneous beating, analogous to a fibrillation. Measurements of direction and velocity of blood flow by making use of a novel system to study heart function in model systems amenable to live imaging revealed a significant correlation between heartbeat arrhythmia and drug treatment, similar to that observed with the same drugs in vertebrates. These results suggest that the heart pacemaker in tunicates may be homologous to that in their vertebrate counterparts in both development and function. |
Databáze: | OpenAIRE |
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