Inhibitors Compounds on Sugarcane Bagasse Saccharification: Effects of Pretreatment Methods and Alternatives to Decrease Inhibition
| Autor: | Gabriela Piccolo Maitan-Alfenas, Túlio Morgan, Valéria Monteze Guimarães, Rafaela I. S. Ladeira-Ázar |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
Formates Formic acid Bioengineering Alkalies Xylose 01 natural sciences Applied Microbiology and Biotechnology Biochemistry Polyethylene Glycols chemistry.chemical_compound Acetic acid Hydrolysis Cellulase Phenols 010608 biotechnology Cellulose Furans Molecular Biology 010405 organic chemistry Chemistry Sulfuric acid General Medicine Saccharum 0104 chemical sciences Glucose Xylanase Carbohydrate Metabolism Bagasse Acids Biotechnology Nuclear chemistry |
| Zdroj: | Applied Biochemistry and Biotechnology. 188:29-42 |
| ISSN: | 1559-0291 0273-2289 |
| DOI: | 10.1007/s12010-018-2900-6 |
| Popis: | Considering bioethanol production, extensive research has been performed to decrease inhibitors produced during pretreatments, to diminish energy input, and to decrease costs. In this study, sugarcane bagasse was pretreated with NaOH, H2SO4, and water. The higher concentration of phenols, 3.3 g/L, was observed in biomass liquid fraction after alkaline pretreatment. Acid pretreatment was responsible to release considerable acetic acid concentration, 2.3 g/L, while water-based pretreatment was the only to release formic acid, 0.02 g/L. Furans derivatives were not detected in liquid fractions regardless of pretreatment. Furthermore, washing step removed most of the phenols from pretreated sugarcane bagasse. Saccharification of alkali-pretreated biomass plus polyethylene glycol (PEG) at 0.4% (w/v) enhanced 8 and 26% the glucose and the xylose release, respectively, while polyvinylpyrrolidone (PVP) also at 0.4% (w/v) increased the release by 10 and 31% of these sugars, respectively, even without washing and filtration steps. Moreover, these polymers cause above 50% activation of endoglucanase and xylanase activities which are crucial for biomass hydrolysis. |
| Databáze: | OpenAIRE |
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