Evaluation of a bovine cathelicidin ELISA for detecting mastitis in the dairy buffalo: Comparison with milk somatic cell count and bacteriological culture
| Autor: | Sergio Uzzau, Paolo Moroni, Vittorio Tedde, Giulia Maria Grazia Puggioni, Maria Filippa Addis, Jacopo Guccione, Paolo Ciaramella, Claudia Pollera, Valerio Bronzo |
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| Přispěvatelé: | Puggioni, Giulia Maria Grazia, Tedde, Vittorio, Uzzau, Sergio, Guccione, Jacopo, Ciaramella, Paolo, Pollera, Claudia, Moroni, Paolo, Bronzo, Valerio, Addis, Maria Filippa |
| Rok vydání: | 2019 |
| Předmět: |
Veterinary medicine
Staphylococcus aureus Buffaloes 040301 veterinary sciences medicine.medical_treatment Cell Count Enzyme-Linked Immunosorbent Assay Mastitis medicine.disease_cause Sensitivity and Specificity Cathelicidin 0403 veterinary science 03 medical and health sciences Cathelicidins medicine Animals Subclinical mastitis 030304 developmental biology 0303 health sciences Subclinical mastitis Water buffalo milk Cathelicidin ELISA Somatic cell count Bacteriological culture General Veterinary biology Diagnostic Tests Routine 04 agricultural and veterinary sciences Staphylococcal Infections biology.organism_classification medicine.disease stomatognathic diseases Milk Herd Cattle Female Bubalus Somatic cell count True positive rate Antimicrobial Cationic Peptides |
| Zdroj: | Research in veterinary science. 128 |
| ISSN: | 1532-2661 |
| Popis: | A recently developed bovine cathelicidin (CATH) ELISA was evaluated in the dairy buffalo (Bubalus bubalis) by testing 618 quarter milk samples from a herd with subclinical mastitis cases. Somatic cell count (SCC) and bacteriological culture (BC) were carried out on the same samples for comparison. Out of 618 quarters, 258 (41.75%) were positive to CATH, 289 (46.76%) had SCC > 200,000 cells/mL, and 457 (73.95%) were positive to BC. The most prevalent microorganism was Staphylococcus aureus (SAU, 35.76% of all quarters), followed by non-aureus staphylococci (NAS, 22.17% of all quarters). Clinical mastitis quarters were only 7 (1.13%). CATH levels were significantly higher in clinical quarters and in high SCC, BC-positive quarters than in healthy, low SCC, BC-negative quarters. The highest median values were observed for SAU and the lowest for NAS. Differences among microorganism classes were generally more significant for SCC than for CATH. Test char acteristics of the CATH ELISA, evaluated by considering as true positives all BC-positive quarters with SCC > 200,000 cells/mL (N = 242), and as true negatives all sterile quarters with SCC < 200,000 cells/mL (N = 44), were as follows: sensitivity 57.85%, specificity 84.09%, positive predictive value 95.24%, negative predictive value 26.62%, accuracy 61.89%. Therefore, the bovine CATH ELISA showed a fair sensitivity and a good specificity in detecting water buffalo mastitis. |
| Databáze: | OpenAIRE |
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