Therapeutic activity of a killer peptide against experimental paracoccidioidomycosis
| Autor: | Luiz R. Travassos, Antonella Salati, Elaine G. Rodrigues, Luis Silva, Walter Magliani, Stefania Conti, Luciano Polonelli |
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| Rok vydání: | 2004 |
| Předmět: |
Pharmacology
Microbiology (medical) Colony-forming unit Paracoccidioides brasiliensis biology Pichia anomala Paracoccidioidomycosis Toxin Mycotoxins biology.organism_classification medicine.disease medicine.disease_cause Yeast In vitro Killer Factors Yeast Recombinant Proteins Microbiology Mice Infectious Diseases In vivo medicine Animals Pharmacology (medical) Oligopeptides |
| Zdroj: | The Journal of antimicrobial chemotherapy. 54(5) |
| ISSN: | 0305-7453 |
| Popis: | OBJECTIVES: To evaluate whether an engineered synthetic decapeptide (KP) derived from the sequence of a recombinant anti-idiotypic antibody, that represents the internal image of a Pichia anomala killer toxin, could be fungicidal in vitro and therapeutic in vivo against Paracoccidioides brasiliensis and paracoccidioidomycosis (PCM). METHODS: Fungicidal activity of KP was assessed in vitro and in vivo by inhibition of colony forming units and by histological examination, 8 days after infection, of organs from mice intravenously injected with a virulent strain of P. brasiliensis (3 x 10(6) yeast cells) and intraperitoneally treated with KP (3.3 microg/g body weight, three doses), in comparison with control animals equally administered with a scrambled decapeptide (SP). RESULTS: KP but not SP was fungicidal in vitro at 39 ng/multiply-budding yeast cell and less efficiently in its D-isomeric form (0.31 microg/multiply-budding yeast cell). It was also able to markedly reduce the fungal load in organs (liver, lung, spleen) of infected animals. CONCLUSIONS: The therapeutic effect observed opens the way for using the antifungal peptide as an alternative control of PCM in association with conventional antifungal drugs. |
| Databáze: | OpenAIRE |
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