A validated UHPLC-MS/MS method for simultaneous quantification of 9 exocyclic DNA adducts induced by 8 aldehydes

Autor: Stéphanie Lagadu, Mathilde Lechevrel, Zeina Dagher, Laurence Galanti, Raphaël Delépée, Héléna Alamil
Přispěvatelé: Aliments Bioprocédés Toxicologie Environnements (ABTE), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU), CHU UCL Namur, Lebanese University [Beirut] (LU), UCL - SSS/IREC/MONT - Pôle Mont Godinne, UCL - (MGD) Laboratoire de biologie clinique
Rok vydání: 2019
Předmět:
Ultrahigh performance liquid chromatography -electrospray ionization- tandem mass spectrometry
Spectrometry
Mass
Electrospray Ionization
Clinical Biochemistry
Pharmaceutical Science
[SDV.CAN]Life Sciences [q-bio]/Cancer
Mass spectrometry
01 natural sciences
Analytical Chemistry
Adduct
chemistry.chemical_compound
DNA Adducts
Analytical method validation
[CHIM.ANAL]Chemical Sciences/Analytical chemistry
Tandem Mass Spectrometry
Drug Discovery
Adductomic
Humans
Crotonaldehyde
Spectroscopy
Chromatography
High Pressure Liquid
Cancer
[SDV.EE.SANT]Life Sciences [q-bio]/Ecology
environment/Health
Aldehydes
Chromatography
010405 organic chemistry
010401 analytical chemistry
Methylglyoxal
Acrolein
Smoking
Acetaldehyde
Exposure biomarkers
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular biology
Middle Aged
3. Good health
0104 chemical sciences
chemistry
Oxidative stress
[SDV.TOX]Life Sciences [q-bio]/Toxicology
Glyoxal
Female
DNA
Biomarkers
Zdroj: Journal of Pharmaceutical and Biomedical Analysis
Journal of Pharmaceutical and Biomedical Analysis, Elsevier, 2020, 179, pp.113007. ⟨10.1016/j.jpba.2019.113007⟩
Journal of pharmaceutical and biomedical analysis, Vol. 179, p. 113007 [1-10] (2020)
ISSN: 1873-264X
0731-7085
Popis: International audience; Human exposure to aldehydes is implicated in several diseases including cancer. These strong electrophilic compounds can react with nucleophilic sites in DNA to form reversible and irreversible modifications. These modifications, if not repaired, can contribute to pathogenesis. The aim of our study was to provide a mass spectrometry (MS)-based profiling method for identifying potential biomarkers of aldehydes exposure. We have developed and validated a highly sensitive method using ultra high performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UHPLC-ESI-MS/MS) for the simultaneous quantitation of 9 exocyclic DNA adducts derived from 8 main exogenous and endogenous aldehydes, namely formaldehyde, acetaldehyde, acrolein, crotonaldehyde, malondialdehyde, 4-hydroxy-2-nonenal, glyoxal and methylglyoxal. Finally, we applied the established method to quantify adducts in genomic DNA isolated from the blood of a smoker and a non-smoker blood samples in order to demonstrate its applicability.
Databáze: OpenAIRE
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