Dynamic imaging of collagen and its modulation in tumors in vivo using second-harmonic generation
| Autor: | Brian Seed, Yves Boucher, Alain Pluen, Trevor D. McKee, Emmanuelle diTomaso, Rakesh K. Jain, Edward B. Brown |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Pathology
medicine.medical_specialty Fibrillar collagen Fibrillar Collagens Dynamic imaging General Biochemistry Genetics and Molecular Biology Pharmacologic intervention Histological staining Mice In vivo Neoplasms Tumor Cells Cultured medicine Animals Humans Collagenases Relaxin Microscopy Confocal Chemistry Second-harmonic generation General Medicine Tumor tissue Elastin Biophysics Neoplasm Transplantation |
| Zdroj: | Nature Medicine. 9:796-800 |
| ISSN: | 1546-170X 1078-8956 |
| DOI: | 10.1038/nm879 |
| Popis: | The content and structure of collagen is essential in governing the delivery of therapeutic molecules in tumors. Thus, simple histological staining of tumor tissue biopsies for collagen could be used to assess the accessibility of molecular therapeutics in tumors. Here we show that it is possible to optically image fibrillar collagen in tumors growing in mice using second-harmonic generation (SHG). Using this noninvasive technique, we estimated relative diffusive hindrance, quantified the dynamics of collagen modification after pharmacologic intervention and provided mechanistic insight into improved diffusive transport induced by the hormone relaxin. This technology could offer basic scientists and clinicians an enhanced ability to estimate the relative penetrabilities of molecular therapeutics. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|