Optimal wavelength for the clot waveform analysis: Determination of the best resolution with minimal interference of the reagents
| Autor: | Julie Laloy, Jonathan Douxfils, Jonathan Evrard, Jean-Michel Dogné, Pauline Thémans, Laure Morimont, Céline Bouvy, Damien Gheldof, François Mullier, Romain Siriez |
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| Přispěvatelé: | UCL - SSS/IREC/MONT - Pôle Mont Godinne, UCL - (MGD) Laboratoire de biologie clinique, UCL - SSS/IREC/MIRO - Pôle d'imagerie moléculaire, radiothérapie et oncologie |
| Rok vydání: | 2018 |
| Předmět: |
Resolution (mass spectrometry)
Clinical Biochemistry 030204 cardiovascular system & hematology Thrombin time Interference (wave propagation) Absorbance 03 medical and health sciences Plasma 0302 clinical medicine medicine Humans Blood Coagulation Prothrombin time Chromatography medicine.diagnostic_test Chemistry Spectrum Analysis Biochemistry (medical) Thrombosis Hematology General Medicine Wavelength Reagent Prothrombin Time Partial Thromboplastin Time Blood Coagulation Tests 030215 immunology Partial thromboplastin time |
| Zdroj: | International journal of laboratory hematology, Vol. 41, no. 3, p. 316-324 (2019) |
| ISSN: | 1751-553X |
| Popis: | Introduction: Clot waveform analysis (CWA), a new methodology to assess coagulation process, can be usefully applied in various clinical settings. However, its clinical use is limited mainly because of the absence of standardization. No consensus exists regarding the wavelengths at which CWA has to be performed what is crucial for the sensitivity of the CWA. Objectives: The primary aim of this study is to determine which wavelength is the most sensitive and specific for CWA. Interindividual baseline absorbance will also be assessed as the impact of reagents from the intrinsic, extrinsic, and common coagulation pathway will be determined. Methods: Plasma samples were screened at wavelengths from 280 to 700 nm to provide absorbance spectra in clotted and nonclotted plasma. The interindividual variability of baseline absorbance was obtained by screening plasma from 50 healthy individuals at 340, 635, and 671 nm. The inner-filter effect of reagents was assessed in plasma or serum when appropriate at the same wavelengths. The reagents were those commonly used for activated partial thromboplastin time, prothrombin time, thrombin time, and dilute Russell's viper venom time. Results: Clotted plasma has higher absorbance value than nonclotted plasma (P 600 nm (P |
| Databáze: | OpenAIRE |
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