Proteomics of the rat gut: analysis of the myenteric plexus-longitudinal muscle preparation
| Autor: | Michael Affolter, Sandrine Wagnière, Laure F. Marvin-Guy, Marie-Claude Courtet-Compondu, Laurent B. Fay, Gabriela Bergonzelli, Luísa V. Lopes, Martin Kussmann |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Proteomics Colon Muscle Proteins Myenteric Plexus Ileum Biology Biochemistry Mass Spectrometry Intestine Small medicine Submucous plexus Animals Electrophoresis Gel Two-Dimensional Rats Long-Evans Molecular Biology Myenteric plexus Two-dimensional gel electrophoresis Muscle Smooth Anatomy Immunohistochemistry Small intestine Cell biology Enzymes Rats medicine.anatomical_structure Peripheral nervous system Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Enteric nervous system |
| Zdroj: | Proteomics. 5(10) |
| ISSN: | 1615-9853 |
| Popis: | The enteric nervous system (ENS)--present all along the gastrointestinal tract - is the largest and most complicated division of the peripheral nervous system that can function independently of the brain. The peripheral nerve cells are organized in two separate but interconnected meshworks, called the myenteric and submucous plexus. The nervous control of intestinal motility is primarily governed by the myenteric plexus (MP), which lies in-between the longitudinal- (LM) and circular-muscle layers and regulates their functions. To determine whether the proteomic technology is adapted to the analysis of specific gut tissues, we dissected the MP-LM layers from the jejunum, ileum, and colon of Long Evans rats, homogenized them, and separated the proteins using two-dimensional gel electrophoresis. A subset of all the visualized protein spots, covering the entire range of molecular weights and isoelectric points, was then selected and further analyzed by matrix-assisted laser desorption/ionization-time of flight and liquid chromatography mass spectrometry. We identified around 80 proteins in each gut segment, and among those, five were segment-specific. Most of the proteins identified were derived from muscle cells, but we also detected some neuron-specific proteins. This study represents, to our knowledge, the first extensive protein catalog of a neuromuscular layer of the rat intestine and it may constitute the basis to understand pathophysiological mechanisms related to the ENS. |
| Databáze: | OpenAIRE |
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