Assessment of the gut bacterial microbiome and metabolome of girls and women with Rett Syndrome
| Autor: | Mohammed Naqvi, Jessica K. Runge, Nabeel Khan, Kathleen J. Motil, Daniel G. Glaze, Miriam Balderas, Kathleen Hoch, Anthony Haag, Alamelu Venkatachalam, Santosh Thapa, Ruth Ann Luna |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Gastrointestinal Diseases
Glutamine Physiology Biochemistry Severity of Illness Index Feces Aromatic Amino Acids RNA Ribosomal 16S Medicine and Health Sciences Medicine Amino Acids Child Multidisciplinary Organic Compounds Acidic Amino Acids Neurochemistry Genomics Neurotransmitters Chemistry Phenotype Medical Microbiology Child Preschool Cohort Physical Sciences Metabolome Female Glutamate Research Article Adult congenital hereditary and neonatal diseases and abnormalities Adolescent Science Rett syndrome Microbial Genomics Microbiology Gas Chromatography-Mass Spectrometry Young Adult Metabolomics Hydroxyl Amino Acids Genetics Rett Syndrome Humans Microbiome Nutrition Bacteria business.industry Gut Bacteria Organic Chemistry Organisms Chemical Compounds Biology and Life Sciences Proteins Sequence Analysis DNA medicine.disease Diet Gastrointestinal Microbiome Metabolism Food Defecation Tyrosine business Dysbiosis Neuroscience |
| Zdroj: | PLoS ONE PLoS ONE, Vol 16, Iss 5, p e0251231 (2021) |
| ISSN: | 1932-6203 |
| Popis: | Background Gastrointestinal problems affect the health and quality of life of individuals with Rett syndrome (RTT) and pose a medical hardship for their caregivers. We hypothesized that the variability in the RTT phenotype contributes to the dysbiosis of the gut microbiome and metabolome in RTT, predisposing these individuals to gastrointestinal dysfunction. Objectives We characterized the gut bacterial microbiome and metabolome in girls and young women with RTT (n = 44) and unaffected controls (n = 21), and examined the relation between the composition of the microbiome and variations in the RTT phenotype. Methods Demographics and clinical information, including growth and anthropometric measurements, pubertal status, symptoms, clinical severity score, bowel movement, medication use, and dietary intakes were collected from the participants. Fecal samples were collected for analysis of the gut microbiome using Illumina MiSeq-based next-generation sequencing of the 16S rRNA gene followed by bioinformatics analysis of microbial composition, diversity, and community structure. Selected end-products of microbial protein metabolism were characterized by liquid chromatography-mass spectrometry. Results The gut bacterial microbiome differed within the RTT cohort based on pubertal status (p Conclusions Although variability in the RTT phenotype contributes to the dysbiosis of the gut microbiome, we presently cannot infer causality between gut bacterial dysbiosis and gastrointestinal dysfunction. Nevertheless, alterations in the gut metabolome may provide clues to the pathophysiology of gastrointestinal problems in RTT. |
| Databáze: | OpenAIRE |
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