Fifteen years of the Protein Crystallography Station: the coming of age of macromolecular neutron crystallography
| Autor: | Clifford J. Unkefer, Julian C.-H. Chen |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Neutron diffraction Neutron scattering 010402 general chemistry 01 natural sciences Biochemistry 03 medical and health sciences enzyme mechanisms neutron crystallography Protein Crystallography Station General Materials Science Neutron Crystallography Los Alamos Neutron Scattering Center Chemistry General Chemistry Oak Ridge National Laboratory Condensed Matter Physics Feature Articles 0104 chemical sciences 030104 developmental biology Beamline QD901-999 X-ray crystallography Neutron source H atoms Spallation Neutron Source |
| Zdroj: | IUCrJ IUCrJ, Vol 4, Iss 1, Pp 72-86 (2017) |
| ISSN: | 2052-2525 |
| DOI: | 10.1107/s205225251601664x |
| Popis: | This article highlights scientific and technical contributions from the Protein Crystallography Station at Los Alamos, the first purpose-built macromolecular crystallography station at a spallation neutron source. The Protein Crystallography Station (PCS), located at the Los Alamos Neutron Scattering Center (LANSCE), was the first macromolecular crystallography beamline to be built at a spallation neutron source. Following testing and commissioning, the PCS user program was funded by the Biology and Environmental Research program of the Department of Energy Office of Science (DOE-OBER) for 13 years (2002–2014). The PCS remained the only dedicated macromolecular neutron crystallography station in North America until the construction and commissioning of the MaNDi and IMAGINE instruments at Oak Ridge National Laboratory, which started in 2012. The instrument produced a number of research and technical outcomes that have contributed to the field, clearly demonstrating the power of neutron crystallography in helping scientists to understand enzyme reaction mechanisms, hydrogen bonding and visualization of H-atom positions, which are critical to nearly all chemical reactions. During this period, neutron crystallography became a technique that increasingly gained traction, and became more integrated into macromolecular crystallography through software developments led by investigators at the PCS. This review highlights the contributions of the PCS to macromolecular neutron crystallography, and gives an overview of the history of neutron crystallography and the development of macromolecular neutron crystallography from the 1960s to the 1990s and onwards through the 2000s. |
| Databáze: | OpenAIRE |
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