Effect of organic cryosolvents on actin structures and actin/[MATH]-actinin binding geometries
| Autor: | E. Pajot-Augy, M. A. V. Axelos |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1993 |
| Předmět: |
Chemistry
Actinin binding General Physics and Astronomy Actinin macromolecular substances Microfilament 01 natural sciences Small-angle neutron scattering 010305 fluids & plasmas law.invention Crystallography law Cytoplasm [PHYS.HIST]Physics [physics]/Physics archives 0103 physical sciences Crystallization Cytoskeleton Actin |
| Zdroj: | Journal de Physique IV Proceedings Journal de Physique IV Proceedings, EDP Sciences, 1993, 03 (C8), pp.C8-241-C8-244. ⟨10.1051/jp4:1993846⟩ |
| ISSN: | 1155-4339 1764-7177 |
| DOI: | 10.1051/jp4:1993846⟩ |
| Popis: | Cryopreservation procedures of living cells involve the use of organic solvents against freezing damage. An efficient cryoprotection might be achieved by obtaining a cytoplasmic gel able to reduce water flux and avoid crystallization. Small angle X-ray scattering experiments were performed at LURE (Orsay, France) to investigate the effects of 1,2- propanediol and glycerol on the structure of actin, a major cytoplasmic protein, and on the binding geometries of actin to [MATH]-actinin, an interconnecting protein. In the presence of 1,2-propanediol, monomeric G-actin exhibits oligomerization into short rod-like structures very close to that of salt-polymerized actin filaments (F-actin). In the presence of [MATH]-actinin. F-actin filaments aggregate into thick and tight bundles. The scattering pattern in the presence of propanediol suggests a "ladder-like" structure with a tilt angle of the [MATH]-actinin molecule relative to F-actin filaments. This angle is even larger with glycerol. The resulting loose structures favor the formation of a microporous network. All these results support previous electron microscopy observations and the structural mechanisms deduced from rheological measurements. |
| Databáze: | OpenAIRE |
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