On the Inverse Correlation of Protein and Oil: Examining the Effects of Altered Central Carbon Metabolism on Seed Composition Using Soybean Fast Neutron Mutants
| Autor: | Cooper Hostetler, Tara Caso, Timothy P. Durrett, Allen Hubbard, Jose A. Aznar-Moreno, Shrikaar Kambhampati, Doug K. Allen, Sally R Bailey |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Endocrinology Diabetes and Metabolism Mutant lcsh:QR1-502 chemistry.chemical_element central carbon metabolism Central carbon metabolism oil 01 natural sciences Biochemistry lcsh:Microbiology Article fast neutron mutants 03 medical and health sciences chemistry.chemical_compound Biosynthesis correlations raffinose family oligosaccharides Food science Raffinose Sugar Molecular Biology Gene 2. Zero hunger soybeans protein linear models seed development fungi food and beverages 030104 developmental biology chemistry Composition (visual arts) Carbon 010606 plant biology & botany |
| Zdroj: | Metabolites Metabolites, Vol 10, Iss 1, p 18 (2019) Metabolites; Volume 10; Issue 1; Pages: 18 |
| ISSN: | 2218-1989 |
| Popis: | Protein and oil levels measured at maturity are inversely correlated across soybean lines; however, carbon is in limited supply during maturation resulting in tradeoffs for the production of other reserves including oligosaccharides. During the late stages of seed development, the allocation of carbon for storage reserves changes. Lipid and protein levels decline while concentrations of indigestible raffinose family oligosaccharides (RFOs) increase, leading to a decreased crop value. Since the maternal source of carbon is diminished during seed maturation stages of development, carbon supplied to RFO synthesis likely comes from an internal, turned-over source and may contribute to the reduction in protein and lipid content in mature seeds. In this study, fast neutron (FN) mutagenized soybean populations with deletions in central carbon metabolic genes were examined for trends in oil, protein, sugar, and RFO accumulation leading to an altered final composition. Two lines with concurrent increases in oil and protein, by combined 10%, were identified. A delayed switch in carbon allocation towards RFO biosynthesis resulted in extended lipid accumulation and without compromising protein. Strategies for future soybean improvement using FN resources are described. |
| Databáze: | OpenAIRE |
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