Distribution of Aβ peptide in whole blood
| Autor: | Peter Seubert, Ruth Motter, J. Randall Slemmon, Sashi Nadanaciva, Ashley Cook, Florentina Catana, Cory L. Painter |
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| Rok vydání: | 2007 |
| Předmět: |
Adult
Male Amyloid beta Clinical Biochemistry Enzyme-Linked Immunosorbent Assay Peptide Biochemistry Analytical Chemistry Pharmacokinetics Blood plasma Humans Distribution (pharmacology) Denaturation (biochemistry) Chromatography High Pressure Liquid Whole blood chemistry.chemical_classification Amyloid beta-Peptides Chromatography biology Chemistry Cell Biology General Medicine Middle Aged Blood proteins biology.protein Female |
| Zdroj: | Journal of Chromatography B. 846:24-31 |
| ISSN: | 1570-0232 |
| DOI: | 10.1016/j.jchromb.2006.08.003 |
| Popis: | The measurement of amyloid beta peptides (Abeta) in blood and plasma is expected to be a useful biomarker as potential therapeutics designed to lower Abeta peptide enter clinical trials. Many reports have suggested that Abeta could bind to substances in blood that may influence the recovery of Abeta peptide in plasma, its detection by conventional ELISAs or the actual turnover and half-life of the peptide in blood. In this study we describe a process for analyzing total Abeta in whole blood and plasma using denaturing solid-phase extraction followed by reverse-phase HPLC linked to ELISA. Comparison of total Abeta peptide levels in whole blood and plasma from the same bleed showed that most of the Abeta peptide is captured in the plasma if the samples are first denatured. In contrast, plasma that was assayed without denaturation could show greater than 70% reduction in apparent total Abeta peptide. This suggested that there was a pool of Abeta peptide in non-denatured plasma that is occluded from detection by ELISA, perhaps by binding to plasma proteins. |
| Databáze: | OpenAIRE |
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