Analysis of mutations in EXT1 and EXT2 in Brazilian patients with multiple osteochondromas
| Autor: | Reinaldo I. Takata, Jaime M. Brum, Savana Camilla Lima Santos, Claudio Sollaci, Carlos E. Speck-Martins, Isabela M. P. O. Rizzo |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Male Osteochondromatosis Adolescent genotype multiple osteochondromas Population Biology N-Acetylglucosaminyltransferases Frameshift mutation 03 medical and health sciences symbols.namesake 0302 clinical medicine Brazilian population Genotype Genetics Prevalence Missense mutation Humans education Child Molecular Biology Genetics (clinical) Aged Sequence Deletion Sanger sequencing education.field_of_study chondrosarcoma Splice site mutation Base Sequence Genetic heterogeneity Point mutation EXT2 Exons Original Articles Middle Aged EXT1 030104 developmental biology 030220 oncology & carcinogenesis Child Preschool Mutation symbols Female Original Article Brazil Exostoses Multiple Hereditary |
| Zdroj: | Molecular Genetics & Genomic Medicine |
| ISSN: | 2324-9269 |
| Popis: | Background Multiple osteochondromas is a dysplasia characterized by growth of two or more osteochondromas. It is genetically heterogeneous, caused by pathogenic variants in EXT1 or EXT2 genes in 70%–90% of patients. The EXT1 is more often mutated than EXT2 gene, with a variable prevalence between populations. There are no data about EXT1 and EXT2 pathogenic variants in patients with multiple osteochondromas in Brazilian population. The aim of this survey is to characterize these to determine the genotype profile of this population. Methods DNA sequencing (Sanger Method) and MLPA analysis were performed to identify point mutations and deletions/duplications in the sample of 153 patients in 114 families. Results Germline variants were identified in 83% of families in which EXT2 variants were detected in 46% and EXT1 in 37% of cases. No variants were detected in 17% of them. We identified 50 different variants, 33 (13 frameshift, 11 nonsense, 5 missense, 2 splice site mutation, and 2 large deletions) in EXT1 and 17 (6 frameshift, 6 splice site mutation, 3 nonsense, 1 missense, and 1 large deletion) in EXT2. Of all 50 variants, 31 (62%) were novel, including 20 out of 33 (60,6%) EXT1 and 11 out of 17 (64.7%) EXT2 alleles. The vast majority of variants (88%) were “loss‐of‐function” and two novel hotspots in EXT2 gene were observed in our study. Conclusion The prevalence of variants detected in the EXT2 gene differs from other researches from Latin America, European, and Asian population. This uncommon prevalence could be related with the newly characterized variant hotspot sites detected in EXT2 gene (p.Ala409Profs*26 and p.Ser290*). A high number of novel variants were also identified indicating that Brazilian population has a unique genetic profile. Characterizing this population and establishing its genotype is essential to understand the molecular pathogenesis of this disease in Brazil. |
| Databáze: | OpenAIRE |
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