Association of different Candida species with catheter‐related candidemia, and the potential antifungal treatments against their adhesion properties and biofilm‐forming capabilities
| Autor: | Xiao-Shu Zuo, Ke Hu, Yanan Liu, Xuan Cai, Liying Zhan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Antifungal Antifungal Agents medicine.drug_class Clinical Biochemistry Microbiology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Cell Adhesion Immunology and Allergy Humans Crystal violet Research Articles Candida Chemistry Biochemistry (medical) Public Health Environmental and Occupational Health Biofilm Micafungin Candidemia Hematology Metabolism Adhesion biochemical phenomena metabolism and nutrition Medical Laboratory Technology adhesion 030104 developmental biology 030220 oncology & carcinogenesis Biofilms Catheter-Related Infections non‐albican Candida Metabolic activity Fluconazole medicine.drug Research Article |
| Zdroj: | Journal of Clinical Laboratory Analysis |
| ISSN: | 1098-2825 0887-8013 |
| Popis: | Background To compare the adhesion properties and biofilm‐forming capabilities of 27 Candida isolates obtained from catheter‐related candidemia patients and to evaluate the inhibitory effects of antifungal agents on different Candida species. Material and Methods Seven C. albicans, six C. parapsilosis, five C. guilliermondii, five C. tropicalis, and four C. glabrata clinical isolates were investigated. We quantified the adherence of these Candida species by flow cytometric method and evaluated the formation of biofilms by XTT reduction and crystal violet methods. Actions of micafungin (MF), fluconazole (FZ), and N‐acetylcysteine (NAC) on the adhesion and biofilm formation of different Candida species were determined. Results Non‐albicans Candida species were demonstrated to have stronger adhesion abilities compared with C. albicans. The biofilm‐forming capabilities of different Candida species were varied considerably, and the degree of biofilm formation might be affected by different assay approaches. Interestingly, C. parapsilosis displayed the highest biofilm formation abilities, while C. glabrata exhibited the lowest total biomass and metabolic activity. Furthermore, the inhibitory activities of MF, FZ, and NAC on fungal adhesion and biofilm formation were evaluated, and the results indicated that MF could reduce the adhesion ability and biofilm metabolism more significantly (p Representation of Candida adhesion profiles. The isolates were characterized by weak, moderate, strong and very strong adhesion patterns. Homogeneous distribution pattern means that yeast cells are attached to an equal amount of microspheres; while heterogeneous distribution pattern indicates that multiple microspheres are bound to a single yeast cell. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text