A supramolecular platform stabilizing growth factors
| Autor: | Simone I. S. Hendrikse, Patricia Y. W. Dankers, E. W. Meijer, Sergio Spaans |
|---|---|
| Přispěvatelé: | Biomedical Materials and Chemistry, Macromolecular and Organic Chemistry |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Polymers and Plastics Polymers medicine.medical_treatment Supramolecular chemistry Bone Morphogenetic Protein 4/chemistry Excipient Bioengineering 02 engineering and technology Bone Morphogenetic Protein 4 Pyrimidinones Article Supramolecular assembly Cell Line Biomaterials 03 medical and health sciences chemistry.chemical_compound Polymers/chemistry Transforming Growth Factor beta Cell Line Tumor Materials Chemistry medicine Humans chemistry.chemical_classification Tumor Protein Stability Growth factor Heparan sulfate Polymer 021001 nanoscience & nanotechnology Supramolecular polymers 030104 developmental biology chemistry Self-healing hydrogels Biophysics 0210 nano-technology Pyrimidinones/chemistry Transforming Growth Factor beta/chemistry medicine.drug Protein Binding |
| Zdroj: | Biomacromolecules, 19(7), 2610-2617. American Chemical Society Biomacromolecules |
| ISSN: | 1525-7797 |
| Popis: | High concentrations of supplemented growth factors can cause oversaturation and adverse effects in in vitro and in vivo studies. Though, these supraphysiological concentrations are often required due to the low stability of growth factors. Here we demonstrate the stabilization of TGF-β1 and BMP4 using supramolecular polymers. Inspired by heparan sulfate, sulfonated peptides were presented on a supramolecular polymer to allow for non-covalent binding to growth factors in solution. After mixing with excipient molecules, both TGF-β1 and BMP4 were shown to have a prolonged half-life compared to the growth factors free in solution. Moreover, high cellular response was measured by a luciferase assay, indicating that TGF-β1 remained highly active upon binding to the supramolecular assembly. The results demonstrate that significant lower concentrations of growth factors can be used when supramolecular polymers bearing growth factor binding moieties are implemented. This approach can also be exploited in hydrogel systems to control growth factor release. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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